Functional analyses unveil the involvement of moso bamboo (Phyllostachys edulis) group I and II NIN-LIKE PROTEINS in nitrate signaling regulation

文献类型： 外文期刊

第一作者： Lin, Zezhong

作者： Lin, Zezhong;Guo, Cuiting;Lou, Shuaitong;Jin, Songsong;Zeng, Weike;Guo, Yanan;Zuo, Zecheng;Ma, Liuyin;Zeng, Weike;Guo, Yanan;Fang, Jun;Xu, Zhenguo;Zuo, Zecheng

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关键词： PeNLPs; Transcription factor; Gene expression; Nitrate signaling; Phyllostachys edulis

期刊名称：PLANT SCIENCE （ 影响因子：3.591； 五年影响因子：4.253 ）

ISSN： 0168-9452

年卷期： 2021 年 306 卷

页码：

收录情况： SCI

摘要： For rapid growth, moso bamboo (Phyllostachys edulis) requires large amounts of nutrients. Nitrate is an indispensable molecular signal to regulate nitrogen absorption and assimilation, which are regulated by group III NINLIKE PROTEINs (NLPs). However, no Phyllostachys edulis NLP (PeNLP) has been characterized. Here, eight PeNLPs were identified, which showed dynamic expression patterns in bamboo tissues. Nitrate did not affect PeNLP mRNA levels, and PeNLP1,-2,-5,-6,-7, and-8 successfully restored nitrate signaling in Arabidopsis atnlp71 protoplasts through recovering AtNiR and AtNRT2.1 expression. Four group I and II PeNLPs (PeNLP1,-2,-5, and-8) interacted with the nitrate-responsive cis-element of PeNiR. Moreover, nitrate triggered the nuclear retention of PeNLP8. PeNLP8 overexpression in Arabidopsis significantly increased the primary root length, lateral root number, leaf area, and dry and wet weight of the transgenic plants, and PeNLP8 expression rescued the root architectural defect phenotype of atnlp7-1 mutants. Interestingly, PeNLP8 overexpression dramatically reduced nitrate content but elevated total amino acid content in Arabidopsis. Overall, the present study unveiled the potential involvement of group I and II NLPs in nitrate signaling regulation and provided genetic resources for engineering plants with high nitrogen use efficiency.

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