Transcriptional profiles of genes potentially involved in extracellular vesicle biogenesis in Schistosoma japonicum

文献类型： 外文期刊

第一作者： Qadeer, Abdul

作者： Qadeer, Abdul;Giri, Bikash Ranjan;Ullah, Hanif;Giri, Bikash Ranjan;Cheng, Guofeng

作者机构：

关键词： Schistosomiasis; Schistosoma japonicum; Extracellular vesicles; Biogenesis; mRNA expression

期刊名称：ACTA TROPICA （ 影响因子：2.555； 五年影响因子：2.496 ）

ISSN： 0001-706X

年卷期： 2021 年 217 卷

页码：

收录情况： SCI

摘要： Schistosomiasis is a severe chronic disease caused by parasitic worms of the genus Schistosoma. Recent studies indicate that schistosomes can secrete extracellular vesicles (EVs), which play important regulatory roles in many biological processes. However, the mechanisms underlying EV biogenesis in schistosomes are poorly understood. In this study, we performed bioinformatic analyses and identified several genes putatively involved in EV biogenesis in Schistosoma japonicum, which were then confirmed by PCR. Quantitative transcriptional profiles of the selected genes indicated that they were differentially expressed in male and female worms as well as in the different developmental stages of S. japonicum. Thus, the highest expression of VAMP3 was detected in cercariae, whereas that of ARF6 was detected in eggs. RAB11A and the Syntenin-encoding gene SDCBP were highly expressed in 14-day schistosomula and VPS4A and RAB27A were highly expressed in 35-day-old adult schistosomes. The expression of RAB11A, CHMP4C, VPS4A, and SDCBP was higher in male worms, whereas that of ARF6, VAMP3, and RAB27A was higher in female worms. Our results are expected to provide important clues for understanding the role of EV biogenesis in S. japonicum development.

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