DCT4-A New Member of the Dicarboxylate Transporter Family in C-4 Grasses

文献类型： 外文期刊

第一作者： Weissmann, Sarit

作者： Weissmann, Sarit;Huang, Pu;Wiechert, Madeline A.;Mockler, Todd C.;Furuyama, Koki;Taniguchi, Mitsutaka;Brutnell, Thomas P.;Schnable, James C.;Huang, Pu

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关键词： DCT4; new transporter gene; grass evolution; C-4 photosynthesis

期刊名称：GENOME BIOLOGY AND EVOLUTION （ 影响因子：3.462； 五年影响因子：3.926 ）

ISSN： 1759-6653

年卷期： 2021 年 13 卷 2 期

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收录情况： SCI

摘要： Malate transport shuttles atmospheric carbon into the CalvinBenson cycle during NADP-ME C-4 photosynthesis. Previous characterizations of several plant dicarboxylate transporters (DCT) showed that they efficiently exchange malate across membranes. Here, we identify and characterize a previously unknown member of the DCT family, DCT4, in Sorghum bicolor. We show that SbDCT4 exchanges malate across membranes and its expression pattern is consistent with a role in malate transport during C-4 photosynthesis. SbDCT4 is not syntenic to the characterized photosynthetic gene ZmDCT2, and an ortholog is not detectable in the maize reference genome. We found that the expression patterns of DCT family genes in the leaves of Zea mays, and S. bicolor varied by cell type. Our results suggest that subfunctionalization, of members of the DCT family, for the transport of malate into the bundle sheath plastids, occurred during the process of independent recurrent evolution of C4 photosynthesis in grasses of the PACMAD clade. We also show that this subfunctionalization is lineage independent. Our results challenge the dogma that key C-4 genes must be orthologues of one another among C-4 species, and shed new light on the evolution of C-4 photosynthesis.

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