Overexpression of an Apocynum venetum flavonols synthetase gene confers salinity stress tolerance to transgenic tobacco plants

文献类型： 外文期刊

第一作者： Wang, Meng

作者： Wang, Meng;Ren, Tingting;Huang, Ruihuan;Li, Yiqiang;Zhang, Chengsheng;Xu, Zongchang;Huang, Ruihuan

作者机构：

关键词： Apocynum venetum; Flavonoids; Gene expression; Germination rate; K+/Na+ ratio; Root growth; Salinity

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：3.72； 五年影响因子：3.966 ）

ISSN： 0981-9428

年卷期： 2021 年 162 卷

页码：

收录情况： SCI

摘要： Soil salinity is a major limiting factor for agricultural production, threatening food security worldwide. A thorough understanding of the mechanisms underlying plant responses is required to effectively counter its deleterious effects on crop productivity. Total flavonoid accumulation reportedly improves salinity tolerance in many crops. Therefore, we isolated the full-length cDNA of a flavonol synthetase (FLS) gene from Apocynum venetum (AvFLS). The gene contained a 1008-bp open reading frame encoding a protein composed of 335 amino acid residues. Multiple sequence alignment showed that the AvFLS protein was highly homologous to FLSs from other plants. AvFLS was expressed in leaves, stems, roots, flowers, and germinated seeds. Expression pattern analysis revealed that AvFLS was significantly induced by salinity stress. AvFLS overexpression in tobacco positively affected the development and growth of transgenic plants under salinity stress: root and seedling growth were inhibited to a lesser extent, while seed germination rate increased. Additionally, the overexpression of AvFLS under salinity stress resulted in an increase in total flavonoid content (1.63 mg g(-1) in wild-type samples and 4.63 mg g(-1) on average in transgenic samples), which accompanied the increase in the activity of antioxidant enzymes and inhibited the production of reactive oxygen species. Further, AvFLS-overexpressing transgenic tobacco plants absorbed more K+ than wild type plants, leading to an increased K+/Na+ ratio, which in turn contributed to the maintenance of Na+/K+ homeostasis. These findings suggest that an AvFLS-induced increase in total flavonoid content enhanced plant salinity tolerance, implying the importance of AvFLS gene responses to salinity stress.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序