LncRNA-FKBP1C regulates muscle fiber type switching by affecting the stability of MYH1B

文献类型： 外文期刊

第一作者： Yu, Jia-ao

作者： Yu, Jia-ao;Wang, Zhijun;Yang, Xin;Ma, Manting;Li, Zhenhui;Nie, Qinghua;Yu, Jia-ao;Wang, Zhijun;Yang, Xin;Ma, Manting;Li, Zhenhui;Nie, Qinghua;Yu, Jia-ao;Wang, Zhijun;Yang, Xin;Ma, Manting;Li, Zhenhui;Nie, Qinghua;Yu, Jia-ao;Wang, Zhijun;Yang, Xin;Ma, Manting;Li, Zhenhui;Nie, Qinghua;Yu, Jia-ao;Wang, Zhijun;Yang, Xin;Ma, Manting;Li, Zhenhui;Nie, Qinghua

作者机构：

期刊名称：CELL DEATH DISCOVERY （ 影响因子：4.114； 五年影响因子：3.977 ）

ISSN：

年卷期： 2021 年 7 卷 1 期

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收录情况： SCI

摘要： Long non-coding RNAs (lncRNAs) are well-known to participate in a variety of important regulatory processes in myogenesis. In our previous RNA-seq study (accession number GSE58755), we found that lncRNA-FKBP1C was differentially expressed between White Recessive Rock (WRR) and Xinghua (XH) chicken. Here, we have further demonstrated that lncRNA-FKBP1C interacted directly with MYH1B by biotinylated RNA pull-down assay and RNA immunoprecipitation (RIP). Protein stability and degradation experiments identified that lncRNA-FKBP1C enhanced the protein stability of MYH1B. Overexpression of lncRNA-FKBP1C inhibited myoblasts proliferation, promoted myoblasts differentiation, and participated in the formation of skeletal muscle fibers. LncRNA-FKBP1C could downregulate the fast muscle genes and upregulate slow muscle genes. Conversely, its interference promoted cell proliferation, repressed cell differentiation, and drove the transformation of slow-twitch muscle fibers to fast-twitch muscle fibers. Similar results were observed after knockdown of the MYH1B gene, but the difference was that the MYH1B gene had no effects on fast muscle fibers. In short, these data demonstrate that lncRNA-FKBP1C could bound with MYH1B and enhance its protein stability, thus affecting proliferation, differentiation of myoblasts and conversion of skeletal muscle fiber types.

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