A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates

文献类型： 外文期刊

第一作者： Zhang, Honghai

作者： Zhang, Honghai;Xie, Xiangming;Zheng, Fei;Zhang, Honghai;Wang, Yuan;Yao, Bin;Luo, Huiying;Brunecky, Roman

作者机构：

关键词： disruptive activity; synergistic effect; hydrolytic; biomass conversion; swollenin

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.235； 五年影响因子：4.926 ）

ISSN： 1664-302X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Swollenins exist within some fungal species and are candidate accessory proteins for the biodegradation of cellulosic substrates. Here, we describe the identification of a swollenin gene, Tlswo, in Talaromyces leycettanus JCM12802. Tlswo was successfully expressed in both Trichoderma reesei and Pichia pastoris. Assay results indicate that TlSWO is capable of releasing reducing sugars from lichenan, barley beta-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin. The specific activity of TlSWO toward lichenan, barley beta-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin is 9.0 +/- 0.100, 8.9 +/- 0.100, 2.3 +/- 0.002 and 0.79 +/- 0.002 U/mg, respectively. Additionally, TlSWO had disruptive activity on Avicel and a synergistic effect with cellobiohydrolases, increasing the activity on pretreated corn stover by up to 72.2%. The functional diversity of TlSWO broadens its applicability in experimental settings, and indicating that it may be a promising candidate for future industrial applications.

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