Deoxymikanolide adversely altered physiology and ultrastructure of Ralstonia solanacearum

文献类型： 外文期刊

第一作者： Li, Yan

作者： Li, Yan;Yang, Xue;Wang, Zhaoguo;Yu, Shuai;Li, Yan;Cao, Ao-cheng;Li, Yan;Yang, Xue;Wang, Zhaoguo;Yu, Shuai;Li, Yan;Yang, Xue;Wang, Zhaoguo;Yu, Shuai;Zeng, Yun-fei;Wang, Zi-yi;Fan, Yan;Pang, Qiuxia

作者机构：

关键词： Mikania micrantha Bunge; Ultrastructural changes; Electric conductivity; Energy metabolism; Intrabacterial ROS and MDA levels

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：2.751； 五年影响因子：3.402 ）

ISSN： 0048-3575

年卷期： 2021 年 174 卷

页码：

收录情况： SCI

摘要： Deoxymikanolide (DEO) was isolated from Mikania micrantha Bunge and identified as a novel antibacterial compound previously. However, the mode of antimicrobial mechanism of DEO was not clear but hypothesized to affect the morphology and physiology of Ralstonia solanacearum cells. In this study, we confirmed our hypothesis via transmission electron microscopy (TEM) observation and comprehensive physiological analyses, including electric conductivity, glycan and phosphorus metabolism, activities of antioxidant enzymes (catalase, peroxidase, and superoxide dismutase), intrabacterial reactive oxygen species (ROS), and malondialdehyde (MDA) levels. We found that glycan and phosphorus metabolism, electric conductivity, intracellular ROS and MDA levels of R. solanacearum cells were significantly increased, while the activities of three antioxidant enzymes were significantly inhibited by DEO treatment. Moreover, TEM analysis showed that DEO treatment led to an earlystage of cell shrinkage, intermediate-stages of cytoplasmic damage, and a final-stage of cell disruption. Altogether, our data presented here indicate that DEO could adversely affect the physiology and morphology of R. solanacearum cells and be treated as an alternative antibacterial treatment in the future.

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