文献类型： 外文期刊
第一作者： Rahman, M. Anisur
作者： Rahman, M. Anisur;Wu, Wei;Yan, Yanchun;Bhuiyan, Shamsul A.;Bhuiyan, Shamsul A.
作者机构：
关键词： sugarcane; drought stress; overexpression; TERF1; physiological and biochemical changes
期刊名称：CROP & PASTURE SCIENCE （ 影响因子：1.57； 五年影响因子：1.792 ）
ISSN： 1836-0947
年卷期： 2021 年 72 卷 4 期
页码：
收录情况： SCI
摘要： Sugarcane (Saccharum hybrid) is an important industrial crop worldwide. Its growth and sucrose contents are severely affected by drought stress. Genetic engineering offers a rapid solution to improve tolerance level of sugarcane against this stress. This study was designed to transform sugarcane with the Tomato ethylene responsive factor 1 (TERF1) gene through Agrobacterium. Embryogenic callus of sugarcane cv. XintaitangR22 was used for transformation with Agrobacterium strain LBA4404 harbouring the pROK2 vector containing the TERF1 gene driven by the CaMV 35S promoter. Highest regeneration efficiency (74%) was obtained with inoculum density (OD600) at 0.4 and co-cultivated for 4 days on MS-based medium; 5.4% transformation efficiency was acquired from the regenerated plants. Successful insertion of the TERF1 gene into sugarcane was indicated by PCR-positive plants (n = 4). Expression of TERF1 transcripts in transgenic lines at various levels was detected by reverse transcriptase-PCR. Under normal conditions, growth status of transgenic lines was similar to that of wild-type plants; by contrast, only transgenic lines were able to withstand water-deficit stress conditions, showing tolerance against drought stress. Physiological and biochemical assays revealed that TERF1-overexpressed plants showed not only increased accumulation of proline, soluble sugars and glycine betaine but also reduced malondialdehyde and H2O2 content in response to drought stress. Our results revealed that overexpression of TERF1 in sugarcane conferred drought tolerance through increased accumulation of osmo-protectant, decreasing reactive oxygen species and malondialdehyde content, which possibly resulted from activation of expression of stress-related genes by TERF1 under stress. These findings indicate that the gene might have a regulatory role in the response to drought stress in sugarcane.
分类号：
- 相关文献
作者其他论文 更多>>
-
Maize height estimation using combined unmanned aerial vehicle oblique photography and LIDAR canopy dynamic characteristics
作者：Liu, Tao;Zhu, Shaolong;Yang, Tianle;Zhang, Weijun;Xu, Yang;Zhou, Kai;Zhao, Yuanyuan;Yao, Zhaosheng;Yang, Guanshuo;Wang, Ying;Sun, Chengming;Liu, Tao;Zhu, Shaolong;Yang, Tianle;Zhang, Weijun;Zhao, Yuanyuan;Yao, Zhaosheng;Yang, Guanshuo;Wang, Ying;Sun, Chengming;Liu, Tao;Zhu, Shaolong;Yang, Tianle;Zhang, Weijun;Zhao, Yuanyuan;Yao, Zhaosheng;Yang, Guanshuo;Wang, Ying;Sun, Chengming;Xu, Yang;Zhou, Kai;Wu, Wei;Wu, Wei;Sun, Jianjun
关键词：Plant height; UAV; LIDAR; Oblique photography; Canopy dynamic characteristics
-
Comparative Analysis of Complete Chloroplast Genomes of Rubus in China: Hypervariable Regions and Phylogenetic Relationships
作者：Xu, Yufen;Li, Yongquan;Chen, Yanzhao;Wang, Longyuan;Xue, Bine;Zhang, Xianzhi;Song, Wenpei;Guo, Wei;Wu, Wei;Xu, Yufen
关键词：Rubus; chloroplast genome; phylogeny; mutation hotspot; infrageneric relationship
-
Unmanned Aerial Vehicle-Scale Weed Segmentation Method Based on Image Analysis Technology for Enhanced Accuracy of Maize Seedling Counting
作者：Yang, Tianle;Zhu, Shaolong;Zhang, Weijun;Zhao, Yuanyuan;Yang, Guanshuo;Yao, Zhaosheng;Liu, Tao;Sun, Chengming;Zhang, Zujian;Yang, Tianle;Zhu, Shaolong;Zhang, Weijun;Zhao, Yuanyuan;Yang, Guanshuo;Yao, Zhaosheng;Liu, Tao;Sun, Chengming;Zhang, Zujian;Song, Xiaoxin;Wu, Wei;Wu, Wei
关键词：image processing; maize seedlings; weed; count; skeleton threshold segmentation
-
New evidence for gut-muscle axis: Lactic acid bacteria-induced gut microbiota regulates duck meat flavor
作者：Xu, Ligen;Xia, Minquan;Wu, Wei;Chen, Jing;Cai, Zhaoxia;Xu, Ligen;Mao, Tingting;Jiang, Chunqing;Zeng, Tao;Tian, Yong;Lu, Lizhi;Mao, Tingting;Jiang, Chunqing
关键词：Duck meat flavor; Gut microbiota; Metabolome; Metagenome; Transcriptome; Gut-muscle axis
-
Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways
作者：Huang, Liang;Lin, Xuanxu;Lu, Lin;Zhang, Liyang;Liao, Xiudong;Cao, Chunyu;Wu, Wei;Luo, Xugang;Liu, Hsiao-Ching;Odle, Jack;See, Miles Todd
关键词：thermal stress; zinc; integrity; barrier function; broiler intestinal epithelial cell
-
Brandt's vole hole detection and counting method based on deep learning and unmanned aircraft system
作者：Wu, Wei;Liu, Shengping;Zhong, Xiaochun;Wu, Wei;Liu, Shengping;Zhong, Xiaochun;Liu, Xiaohui;Wang, Dawei;Wang, Dawei;Lin, Kejian;Wang, Dawei
关键词：pest rodent monitoring; vole hole detection; unmanned aerial vehicles; deep learning; threshold optimization
-
The elite haplotype OsGATA8-H coordinates nitrogen uptake and productive tiller formation in rice
作者：Wu, Wei;Dong, Xiaoou;Chen, Gaoming;Lin, Zhixi;Chi, Wenchao;Tang, Weijie;Yu, Jun;Wang, Saisai;Jiang, Xingzhou;Liu, Xiaolan;Wu, Yujun;Wang, Chunyuan;Cheng, Xinran;Wang, Chunming;Wan, Jianmin;Cheng, Xinran;Wang, Haiyang;Wan, Jianmin;Cheng, Xinran;Wang, Chunming;Zhang, Wei;Xuan, Wei;Terzaghi, William;Ronald, Pamela C.;Ronald, Pamela C.;Ronald, Pamela C.
关键词：
