Ionic liquid extraction of silkworm pupa protein and its biological characteristics

文献类型： 外文期刊

第一作者： Zeng, Qing-Lei

作者： Zeng, Qing-Lei;Zhang, Ning;Zhang, Yue-Yue;Xin, Xiang-Dong;Attaribo, Thomas;Shao, Ying;Tang, Liu-Mei;Zhang, Ran;Gui, Zhongzheng;Zhang, Ran;Gui, Zhongzheng;Lee, Kwang Sik;Jin, Byung Rae

作者机构：

关键词： Bombyx mori; Pupa protein; Ionic liquid; Extraction; Characteristics

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.101； 五年影响因子：1.1 ）

ISSN： 1226-8615

年卷期： 2021 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Silkworm (Bombyx mori) pupa protein (SPP) is a high-quality source of animal protein with substantial nutritional benefits and health value. To develop an efficient extraction method for SPP that is environmentally friendly, we selected choline hydroxide ionic liquid (CH-IL) as the extraction solvent and performed orthogonal experiments to optimize the extraction conditions. We demonstrated that 3% CH-IL, a solid-to-liquid ratio (g/mL) of 1:30, an extraction temperature of 40 degrees C, and an extraction time of 1 h facilitated the most efficient extraction. Compared to the conventional alkali solubilization-acid precipitation method, the CH-IL extraction increased protein content by 12.14%. Protein structure analysis showed that the beta-sheet content increased by 10.98% and that of disulfide bonds reduced by 16.4%. The processing properties of the CH-IL extracted protein showed that the solubility, emulsification, and foaming capacity were enhanced by 82.87%, 15.44%, and 18.97%, respectively. The physical properties of SPP remarkably improved relative to the increased stretching of the polypeptide chains. The findings of this study provide technical knowledge that will enhance the processing performance of pupal proteins.

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