mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2

文献类型： 外文期刊

第一作者： Li, Jinpeng

作者： Li, Jinpeng;Wang, Yuxin;Du, Yanbin;Fan, Qingying;Sun, Liyun;Wang, Yang;Li, Jinpeng;Wang, Yuxin;Du, Yanbin;Fan, Qingying;Sun, Liyun;Wang, Yang;Zhang, Hui;Yi, Li;Wang, Shaohui

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关键词： Streptococcus suis; luxS gene; Acid resistance; Iron stress; Oxidative stress

期刊名称：BMC MICROBIOLOGY （ 影响因子：2.989； 五年影响因子：3.381 ）

ISSN： 1471-2180

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. We have previously reported the structure of LuxS protein and found that the luxS gene is closely related to biofilm, virulence gene expression and drug resistance of SS2. However, the mechanism of luxS mediated SS2 stress response is unclear. Therefore, this experiment performed stress response to luxS mutant (Delta luxS) and complement strain (C Delta luxS), overexpression strain (luxS+) and wild-type SS2 strain HA9801, and analyzed the differential phenotypes in combination with transcriptome data. Results The results indicate that the luxS gene deletion causes a wide range of phenotypic changes, including chain length. RNA sequencing identified 278 lx-regulated genes, of which 179 were up-regulated and 99 were down-regulated. Differential genes focus on bacterial growth, stress response, metabolic mechanisms and drug tolerance. Multiple mitotic genes were down-regulated; while the ABC transporter system genes, cobalamin /Fe3+-iron carrier ABC transporter ATPase and oxidative stress regulators were up-regulated. The inactivation of the luxS gene caused a significant reduction in the growth and survival in the acid (pH = 3.0, 4.0, 5.0) and iron (100 mM iron chelator 2,2 '-dipyridyl) stress environments. However, the mutant strain Delta luxS showed increased antioxidant activity to H2O2 (58.8 mmol/L). Conclusions The luxS gene in SS2 appears to play roles in iron metabolism and protective responses to acidic and oxidative environmental conditions.

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