lncRNA DMRT2-AS acts as a transcriptional regulator of dmrt2 involving in sex differentiation in the Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Feng, Bo

作者： Feng, Bo;Zhang, Zhihua;Feng, Bo;Li, Shuo;Wang, Qian;Tang, Lili;Zhang, Zhihua;Shao, Changwei;Wang, Qian;Tang, Lili;Shao, Changwei;Li, Shuo;Huang, Fei;Mahboobe, Shahid

作者机构：

关键词： Long non-coding RNA; dmrt2; Sexual development; Cynoglossus semilaevis; Epigenetics

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.219； 五年影响因子：2.046 ）

ISSN： 1096-4959

年卷期： 2021 年 253 卷

页码：

收录情况： SCI

摘要： Long non-coding RNAs (lncRNAs) contribute to various biological processes, including sexual development. As a member of the DMRT family, dmrt2 plays a very important role in sex determination and differentiation. In this study, we cloned and characterized the lncRNA DMRT2-AS (referred to as dmrt2 antisense) associated with dmrt2 from the gonads of the Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of DMRT2-AS was 537 bp. Based on a sequence alignment, DMRT2-AS overlapped with dmrt2 in reverse on exon 4 and intmn 3, with a region of overlap of 221 bp on exon 4. RT-qPCR showed that DMRT2-AS was highly expressed in the testis of Chinese tongue sole. In addition, the expression of DMRT2-AS increased continuously during male gonadal development. In vitro experiments and bioinformatics predictions showed that DMRT2-AS promoted the expression of dmrt2 at the transcriptional level. These results suggest that DMRT2-AS acts as a transcriptional regulator of dmrt2 and plays an important role in the gonadal differentiation of male.

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