Slr0320 Is Crucial for Optimal Function of Photosystem II during High Light Acclimation in Synechocystis sp. PCC 6803

文献类型： 外文期刊

第一作者： Zhang, Hao

作者： Zhang, Hao;Zhang, Ye;Zhang, Pengpeng;Ge, Haitao;Wang, Yingchun

作者机构：

关键词： high light acclimation; photosystem II; electron transfer between Q(A) and Q(B); low molecular weight subunits; quantitative proteomics; Synechocystis sp; PCC 6803

期刊名称：LIFE-BASEL （ 影响因子：2.991； ）

ISSN：

年卷期： 2021 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Upon exposure of photosynthetic organisms to high light (HL), several HL acclimation responses are triggered. Herein, we identified a novel gene, slr0320, critical for HL acclimation in Synechocystis sp. PCC 6803. The growth rate of the Delta slr0320 mutant was similar to wild type (WT) under normal light (NL) but severely declined under HL. Net photosynthesis of the mutant was lower under HL, but maximum photosystem II (PSII) activity was higher under NL and HL. Immunodetection revealed the accumulation and assembly of PSII were similar between WT and the mutant. Chlorophyll fluorescence traces showed the stable fluorescence of the mutant under light was much higher. Kinetics of single flash-induced chlorophyll fluorescence increase and decay revealed the slower electron transfer from Q(A) to Q(B) in the mutant. These data indicate that, in the Delta slr0320 mutant, the number of functional PSIIs was comparable to WT even under HL but the electron transfer between Q(A) and Q(B) was inefficient. Quantitative proteomics and real-time PCR revealed that expression profiles of psbL, psbH and psbI were significantly altered in the Delta slr0320 mutant. Thus, Slr0320 protein plays critical roles in optimizing PSII activity during HL acclimation and is essential for PSII electron transfer from Q(A) to Q(B).

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序