Anti-Inflammatory Effects and Mechanisms of Dandelion in RAW264.7 Macrophages and Zebrafish Larvae

文献类型： 外文期刊

第一作者： Li, Wenju

作者： Li, Wenju;Luo, Fulong;Fan, Bei;Yang, Mingran;Guan, Dongyan;Wang, Fengzhong;Wang, Qiong;Li, Wenju;Luo, Fulong;Zhong, Wu;Wu, Xiaohui;Zhong, Wu;Wang, Qiong

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关键词： dandelion extract (DE); RAW264; 7 cells; zebrafish larvae; M1; M2 subtype; inflammation; apoptosis

期刊名称：FRONTIERS IN PHARMACOLOGY （ 影响因子：5.988； 五年影响因子：6.455 ）

ISSN：

年卷期： 2022 年 13 卷

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收录情况： SCI

摘要： Dandelions (Taraxacum spp.) play an important role in the treatment of inflammatory diseases. In this study, we investigated the anti-inflammatory effects of Dandelion Extract (DE) in LPS-induced RAW264.7 macrophages and copper sulfate (CuSO4)-induced zebrafish larvae. DE was not toxic to RAW264.7 cells at 75 mu g/ml as measured by cell viability, and DE inhibited LPS-induced cell morphological changes as measured by inverted microscopy. In survival experiments, DE at 25 mu g/ml had no toxicity to zebrafish larvae. By using an enzymatic standard assay, DE reduced the production of nitric oxide (NO) in LPS-induced RAW264.7 cells. Fluorescence microscopy results show that DE reduced LPS-induced ROS production and apoptosis in RAW264.7 cells. DE also inhibited CuSO4-induced ROS production and neutrophil aggregation in zebrafish larvae. The results of flow cytometry show that DE alleviated the LPS-induced cell cycle arrest. In LPS-induced RAW264.7 cells, RT-PCR revealed that DE decreased the expression of M1 phenotypic genes iNOS, IL-6, and IL-1 beta while increasing the expression of M2 phenotypic genes IL-10 and CD206. Furthermore, in CuSO4-induced zebrafish larvae, DE reduced the expression of iNOS, TNF-alpha, IL-6, and IL-10. The findings suggest that DE reduces the LPS-induced inflammatory response in RAW264.7 cells by regulating polarization and apoptosis. DE also reduces the CuSO4-induced inflammatory response in zebrafish larvae.

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