EFFECTS OF CRYOPRESERVATION ON ACROSIN ACTIVITY AND DNA DAMAGE OF RUSSIAN STURGEON (Acipenser gueldenstaedtii) SEMEN

文献类型： 外文期刊

第一作者： Zhuang, Ping

作者： Zhuang, Ping

作者机构：

关键词： Acipenser gueldenstaedtii; acrosin activity; cryopreservation; DNA damage; semen

期刊名称：CRYOLETTERS （ 影响因子：0.702； 五年影响因子：0.838 ）

ISSN： 0143-2044

年卷期： 2021 年 42 卷 3 期

页码：

收录情况： SCI

摘要： BACKGROUND: Cryopreservation of sturgeon sperm can be successful, but there can be a decrease in sperm viability and the reasons are not clear. OBJECTIVE: To investigate variations in the acrosin activity and the DNA integrity of Acipenser gueldenstaedtii semen during cryopreservation at -196 degrees C. MATERIALS AND METHODS: Fish semen samples were randomly divided into three groups: [1] fresh control; [2] native semen diluted 1:1 with 23.4 mM sucrose + 0.25 mM KCl + 30 mM Tris (pH 8.0) and the addition of 10% methanol as cryoprotectant; and [3] semen without any diluents or cryoprotectants. Acrosin activity and DNA damage (COMET assay) was assessed. RESULTS: The average acrosin activity fell to 61% and 27% of the control for cryoprotected and non-cryoprotected semen after cryopreservation. The differences among the three groups were significant (P<0.05). We also observed that various indexes of DNA damage (L-tail; tail DNA, tail momentum, olive tail momentum) were higher in semen that had been frozen. CONCLUSION: Although cryopreservation of semen induces decreased acrosin activity and increased DNA damage, cryoprotectants can protect the semen during cryopreservation.

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