Ultrasensitive immunoassay for detection of zearalenone in agro-products using enzyme and antibody co-embedded zeolitic imidazolate framework as labels

文献类型： 外文期刊

第一作者： Liu, Zhenjiang

作者： Liu, Zhenjiang;Dong, Fengshou;Li, Yuanbo;Liu, Xingang;Xu, Jun;Wu, Xiaohu;Zheng, Yongquan;Liu, Zhenjiang;Wang, Xinwei;Guo, Yanguo

作者机构：

关键词： Ultrasensitive; Immunoassay; Zearalenone; HRP; Ab@ZIF-L; Agro-products

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：9.038； 五年影响因子：8.512 ）

ISSN： 0304-3894

年卷期： 2021 年 412 卷

页码：

收录情况： SCI

摘要： Zearalenone (ZEN) has a potential hazard to human health, and is frequently found in agro-products. To minimize ZEN exposure to consumers, a novel metal-organic framework-based immunoassay system using zeolitic imidazolate framework-encapsulated horseradish peroxidase and goat anti-mouse IgG (HRP/Ab@ZIF-L) as labels was proposed for rapid and ultrasensitive detection of ZEN in agro-products. The HRP/Ab@ZIF-L not only maintained recognition ability of antibody and catalytic activity of enzyme, but also protected encapsulated proteins against high temperature, organic solvents and long term storage. Under optimal conditions, the detection limit of HRP/Ab@ZIF-L-based immunoassay reached 0.5 ng/L for ZEN, which was approximately 126fold lower than that of conventional HRP-based immunoassay. Moreover, the proposed method showed an excellent selectivity, and a good dynamic linear detection for ZEN in the range of 0.5 ng/L to 0.476 ?g/L. The recoveries of ZEN from spiked corn and wheat samples ranged from 84.50% to 96.70% with the relative standard deviation under 8.9%. In brief, the proposed immunoassay method has potential application for rapid and ultrasensitive detection of ZEN in agro-products.

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