Expression of Arabidopsis Ornithine Aminotransferase (AtOAT) encoded gene enhances multiple abiotic stress tolerances in wheat
文献类型： 外文期刊
第一作者： Anwar, Alia
作者： Anwar, Alia;Wang, Ke;Wang, Jing;Shi, Lei;Du, Lipu;Ye, Xingguo
作者机构：
关键词： Abiotic stress tolerance; Ornithine aminotransferase; Ornithine pathway; Proline biosynthesis; Triticum aestivum
期刊名称：PLANT CELL REPORTS （ 影响因子：3.825； 五年影响因子：3.686 ）
ISSN： 0721-7714
年卷期：
页码：
收录情况： SCI
摘要： Key message The drought and salt tolerances of wheat were enhanced by ectopic expression of the Arabidopsis ornithine aminotransferase (AtOAT) encoded gene. The OAT was confirmed to play a role in proline biosynthesis in wheat. Proline (Pro) accumulation is a common response to both abiotic and biotic stresses in plants. Ornithine aminotransferase (OAT) is pyridoxal-5-phosphate dependent enzyme involved in plant proline biosynthesis. During stress condition, proline is synthesized via glutamate and ornithine pathways. The OAT is the key enzyme in ornithine pathway. In this study, an OAT gene AtOAT from Arabidopsis was expressed in wheat for its functional characterization under drought, salinity, and heat stress conditions. We found that the expression of AtOAT enhanced the drought and salt stress tolerances of wheat by increasing the proline content and peroxidase activity. In addition, it was confirmed that the expression of AtOAT also played a partial tolerance to heat stress in the transgenic wheat plants. Moreover, quantitative real-time PCR (qRT-PCR) analysis showed that the transformation of AtOAT up-regulated the expression of the proline biosynthesis associated genes TaOAT, TaP5CS, and TaP5CR, and down-regulated that of the proline catabolism related gene TaP5CDH in the transgenic plants under stress conditions. Moreover, the genes involved in ornithine pathway (Orn-OAT-P5C/GSA-P5CR-Pro) were up-regulated along with the up-regulation of those genes involved in glutamate pathway (Glu-P5CS-P5C/GSA-P5CR-Pro). Therefore, we concluded that the expression of AtOAT enhanced wheat abiotic tolerance via modifying the proline biosynthesis by up-regulating the expression of the proline biosynthesis-associated genes and down-regulating that of the proline catabolic gene under stresses condition.
分类号：
- 相关文献
作者其他论文 更多>>
-
Heavy rainfall stimulates more N2O emissions from wheat fields during basal and overwintering fertilization phases
作者：Wang, Jing;Liu, Qiang;Liu, Qiang;Zhao, Suya;He, Shiying;Yang, Bei;Wang, Ning;Hou, Pengfu;Xue, Lihong;Yang, Linzhang;Zhao, Suya;He, Shiying;Wang, Ning;Hou, Pengfu;Xue, Lihong
关键词：Rainfall; Nitrous oxide; Nitrogen transformation; Functional microorganisms
-
Soil bacterial diversity and community structure of Suaeda glauca vegetation in the Hetao Irrigation District, Inner Mongolia, China
作者：Dong, Ruixiao;Wang, Xinbo;Li, Yuyi;Zhang, Hongyuan;Li, Xiaobin;Song, Jiashen;Chang, Fangdi;Feng, Wenhao;Pang, Huancheng;Wang, Jing
关键词：Suaeda glauca; saline-alkali soil; rhizosphere; bacteria; community structure
-
Identification of candidate gene associated with maize northern leaf blight resistance in a multi-parent population
作者：Bi, Yaqi;Jiang, Fuyan;Yin, Xingfu;Shaw, Ranjan K.;Guo, Ruijia;Wang, Jing;Fan, Xingming
关键词：Zea mays; Northern leaf blight; Multi-parent population; Genome-wide association analysis; QTL mapping; CATETO germplasm
-
Integrating physical model-based features and spatial contextual information to estimate building height in complex urban areas
作者：Dong, Baiyu;Huang, Chenhao;Tong, Cheng;Li, Sinan;Deng, Jinsong;Wang, Ke;Zheng, Qiming;Zheng, Qiming;Lin, Yue;Chen, Binjie;Ye, Ziran
关键词：Building Height Estimation; Urbanization; Machine Learning; Physical Model -Based Features; Spatial Contextual Information
-
Full-length transcriptome and RNA-Seq analyses reveal the resistance mechanism of sesame in response to Corynespora cassiicola
作者：Jia, Min;Ni, Yunxia;Zhao, Hui;Liu, Xintao;Yan, Wenqing;Zhao, Xinbei;Wang, Jing;He, Bipo;Liu, Hongyan;Jia, Min;Ni, Yunxia;Zhao, Hui;Liu, Hongyan
关键词：Sesame; full-length transcriptome; WGCNA; resistant; susceptible; Corynespora cassiicola
-
Inhibition of Hyperglycemia and Hyperlipidemia by Blocking Toll-like Receptor 4: Comparison of Wild-Type and Toll-like Receptor 4 Gene Knockout Mice on Obesity and Diabetes Modeling
作者：Zhao, Xingyu;Huang, Wuyang;Zhao, Xingyu;Zheng, Jiawei;Huang, Wuyang;Wang, Jing;Huang, Wuyang;Li, Bin
关键词：TLR4; glycolipid metabolism; obesity and diabetes modeling; knockout
-
Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance
作者：Liu, Jintao;Li, Dianjue;Wang, Jing;Wang, Qian;Fu, Qi;Zhu, Guangtao;Liu, Jintao;Zhu, Guangtao;Guo, Xiao;Yang, Xiaohui;Kear, Philip
关键词：Potato; CPA; NaCl stress; Gene expression; Ion transporter
