Novel fluorescence immunoassay for the detection of zearalenone using HRP-mediated fluorescence quenching of gold-silver bimetallic nanoclusters

文献类型： 外文期刊

第一作者： Liu, Zhenjiang

作者： Liu, Zhenjiang;Wang, Xiaoyan;Li, Weibin;Sun, Jianfan;Wang, Xinwei;Huang, Yuqian;Guo, Yanguo;Sun, Jianfan;Ren, Xuexiang;Zeng, Huawei

作者机构：

关键词： Ratiometric fluorescence ELISA; Gold-silver bimetallic nanoclusters; Horseradish peroxidase; Zearalenone

期刊名称：FOOD CHEMISTRY （ 影响因子：6.306； 五年影响因子：6.219 ）

ISSN： 0308-8146

年卷期： 2021 年 355 卷

页码：

收录情况： SCI

摘要： In the presented study, a horseradish peroxidase (HRP)-mediated ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) for zearalenone (ZEN) was reported based on fluorescence quenching of goldsilver bimetallic nanoclusters (Au-Ag NCs). HRP-antibody was used as a bridge in this immunoassay, linking the ratiometric fluorescence signal to the ZEN concentration. HRP catalyzed the oxidization of o-phenylenediamine in the presence of H2O2, leading to the formation of 2,3-diaminophenazine, which not only delivered a new peak at 580 nm but also quenched Au-Ag NCs fluorescence at 690 nm. Under optimal conditions, the detection limit for the proposed ELISA was 0.017 ng/mL, which was approximately 6.6-fold lower than conventional ELISA. Moreover, analytical performances were evaluated fully including specificity, accuracy, precision, and practicability, and showed that this method provides a potential platform for sensitive and reliable detection of ZEN.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序