文献类型： 外文期刊
第一作者： Song, Chang
作者： Song, Chang;Chen, Wenhui;Kuang, Jingyu;Yao, Yao;Tang, Sheng;Shen, Wei;Zhao, Ze;Guo, Xijie;Zhao, Ze;Guo, Xijie;Lee, Hian Kee
作者机构：
关键词： Multiple microRNAs; Simultaneous detection; Signal differentiation; Spatio-temporal separation; MicroRNA probe
期刊名称：TRAC-TRENDS IN ANALYTICAL CHEMISTRY （ 影响因子：9.801； 五年影响因子：9.661 ）
ISSN： 0165-9936
年卷期： 2021 年 139 卷
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) are a class of endogenous, noncoding, single-stranded RNA molecules with an average length of similar to 22 nucleotides. Various miRNAs play important roles in the processes of cell differentiation, biological development, and disease development. More often, these roles are collectively played by a group of miRNAs rather than a single miRNA. Analytical tools that only quantify a single miRNA are inadequate in meeting the needs of miRNA research. Therefore, based on different signal differentiation techniques in conjunction with nucleic acid amplification strategies, a variety of methods for simultaneous detection of multiple miRNAs have been developed. This article summarizes the latest progress in the simultaneous detection of multiple miRNAs, and discusses the applications of these methods. This review also provides some ideas for the further development, focusing on the signal amplification and discrimination strategies demonstrated by some methods, as well as the application and future developmental trends of these methods. (C) 2021 Elsevier B.V. All rights reserved.
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