Identification of reference genes for gene expression analysis at different developmental stages of the bumblebee Bombus terrestris (Hymenoptera: Apidae)
文献类型： 外文期刊
第一作者： Qin, Haoran
作者： Qin, Haoran;Dong, Kun;He, Shaoyu;Qin, Haoran;Huang, Jiaxing;Wu, Jie
作者机构：
关键词： bumblebee; Bombus terrestris; development stage; reference gene; qRT-PCR
期刊名称：APIDOLOGIE （ 影响因子：1.828； 五年影响因子：2.444 ）
ISSN： 0044-8435
年卷期：
页码：
收录情况： SCI
摘要： Bumblebees are important pollinators for many wild plants and crops. To explore gene expression characteristics in depth, stable reference genes will be essential across different developmental stages. In this context, we evaluated the stability of eight common reference genes at ten developmental stages in the bumblebee Bombus terrestris (B. terrestris) with four algorithms and two rank approaches. The results showed that all the primers for the reference genes exhibited high specificity, linear correlation (R-2-value 0.9917 similar to 0.9978) and a good PCR amplification efficiency (E-value 95.4 similar to 108.6%). The combined use of RPL13 and RP49 was suggested to be the most suitable reference genes. Our candidate reference genes will help to improve the precision of the detection of gene expression at different developmental stages of bumblebee species.
分类号：
- 相关文献
作者其他论文 更多>>
-
Telomere-to-telomere genome assembly of eggplant ( Solanum melongena L.) promotes gene fine localization of the green stripe (GS) in pericarp
作者：Fang, Huarong;Wu, Jie;Xie, Liang;Li, Yizhiqi;Huang, Jingjing;Yan, Xiaoyun;He, Xuehan;Deng, Wentao;Li, Ruirui;Yu, Wenjin;Wang, Peng;Chen, Jie;Ji, Yanhai;Wen, Changlong
关键词：Eggplant; T2T genome; Green stripe
-
Novel 5-alkenylthiothiazolidinone scaffold discovery as Multi-Chitinases inhibitors to arrest the molting of Asian corn borer
作者：Shi, Dongmei;Ji, Xinge;Zou, Renxuan;Jiang, Zhiyang;Han, Qing;Huang, Jiaxing;Duan, Hongxia;Shi, Dongmei;Ji, Xinge;Zou, Renxuan;Jiang, Zhiyang;Han, Qing;Huang, Jiaxing;Duan, Hongxia;Xu, Zhijian;Duan, Yangwei;Yang, Qing;Duan, Yangwei;Yang, Qing
关键词：5-alkenylthiothiazolidinone scaffold; Multichitinase inhibitor; Amide bond; Asian corn borer; insect growth regulator
-
The control effect and induced disease resistance mechanism of Bacillus tequilensis on wheat powdery mildew
作者：Bi, Qiuyan;Lu, Fen;Wu, Jie;Liu, Xiangyu;Han, Xiuying;Zhao, Jianjiang;Bi, Qiuyan;Lu, Fen;Wu, Jie;Liu, Xiangyu;Han, Xiuying;Zhao, Jianjiang
关键词：Bacillus tequilensis; Blumeria graminis; Systemic acquired resistance
-
Specialized metabolites present in Camellia reticulata nectar inhibit the growth of nectar-inhabiting microorganisms
作者：Xun, Lijie;Huang, Rong;Meng, Qingxin;Wu, Xiaoman;Gong, Xueyang;Dong, Kun;Xun, Lijie;Li, Qiongyan;Su, Rui;Zhang, Renbin;Li, Linshu
关键词：
Camellia reticulata ; nectar; nectar microorganisms; nectar metabolites; antibacterial; homeostasis -
Flupyradifurone Exhibits Greater Toxicity to the Asian Bumblebee Bombus lantschouensis Compared to the European Bumblebee Bombus terrestris
作者：Jie, Chunting;Zhang, Hong;Zhou, Ziyu;Han, Bo;Guo, Baodi;Guo, Yi;Hu, Xiao;Iqbal, Shahid;Wei, Bingshuai;Huang, Jiaxing;Dai, Pingli;An, Jiandong;Miao, Zhengying
关键词：bumblebee; acute toxicity; chronic toxicity; risk assessment
-
Mating Frequencies of Eastern Honey Bee (Apis cerana) Queens Produced in Different Periods of Queen-Producing Season
作者：Yuan, Chunying;Tang, Jiao;Ding, Guiling;Huang, Jiaxing
关键词：mating frequency; heterozygosity; inbreeding coefficient; queen;
Apis cerana -
Influence of Different Diets on Growth and Development of Eastern Honey Bee (Apis cerana)
作者：Liang, Ruonan;Huang, Jiaxing;Ding, Guiling;Liang, Cheng;Zhang, Yi
关键词：pollen; pollen substitute; diet consumption; body weight; survival; hypopharyngeal gland; midgut proteolytic enzyme activity
