Anti-idiotypic single-chain variable fragment antibody partially mimic the functionally spatial structure of Cry2Aa toxin

文献类型： 外文期刊

第一作者： Lin, Manman

作者： Lin, Manman;Liu, Yuan;Zhang, Xiao;Zhong, Jianfeng;Hu, Xiaodan;Xu, Chongxin;Xie, Yajing;Zhang, Cunzheng;Liang, Ying;Liu, Xianjin;Lin, Manman;Hu, Xiaodan;Xie, Yajing;Lin, Johnson;Liu, Yuan

作者机构：

关键词： Cry2Aa toxin; Anti-idiotypic antibody; Single-chain variable fragment (scFv)

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：2.877； 五年影响因子：2.549 ）

ISSN： 0003-2697

年卷期： 2021 年 625 卷

页码：

收录情况： SCI

摘要： The anti-idiotypic antibody is widely used in the field of immunology to simulate structural features or even induce the biological activity of antigens. In this study, we obtained seven anti-idiotypic single-chain variable fragments (scFv) antibodies of Cry2Aa toxin from a phage-displayed mutant library constructed using errorprone PCR technique. A mutant designated 2-12B showed the best binding ability amongst all anti-idiotypic scFv isolates to Plutella xylostella brush border membrane vesicles (BBMVs). 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs. Homology modeling and molecular docking demonstrated that 2-12B and Cry2Aa toxin have seven common binding amino acid residues in polycalin. Insect bioassay results suggested that 2-12 had insecticidal efficacy against P. xylostella larvae. These results indicated that the Cry2Aa anti-idiotypic scFv antibody 2-12B partially mimicked the structure and function of Cry2Aa toxin. The anti-idiotypic scFv antibody provides the basic material for the future study of surrogate molecules or new insecticidal materials.

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