BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

文献类型： 外文期刊

第一作者： Li, Yanfang

作者： Li, Yanfang;Jiao, Yubing;Xie, Jingjing;Yin, Jing;Zhao, Xiuxiang;Shi, Jia;Chen, Huamin

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关键词： BLB8; tobacco mosaic virus; antiviral activity; Brevibacillus laterosporus; immune response

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.75； 五年影响因子：3.861 ）

ISSN： 1526-498X

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收录情况： SCI

摘要： BACKGROUND Tobacco mosaic virus (TMV) is one of destructive plant viruses, causing serious economic losses in the world. Using antiviral proteins or elicitors to inhibit viral infection or promote plant immunity is one of the efficient strategies against TMV. Our previous study identified that the fermentation broth of Brevibacillus laterosporus strain B8 showed strong antiviral activity against TMV. However, the active antiviral ingredient is still unclear. RESULTS Here, BLB8 (B. laterosporus strain B8 protein, BLB8), an antiviral protein from B. laterosporus strain B8 was isolated and characterized. BLB8 showed protective, inactive and curative effects against TMV, and the inhibition rate reached up to 63%, 83% and 55%, respectively. BLB8 infiltrated around the infection site of the recombinant virus TMV-GFP inhibited the systemic extend and movement of TMV. Pretreatment of the bottom leaves with BLB8 inhibited the spread and accumulation of TMV in upper systemic leaves. Furthermore, BLB8 caused hypersensitive response (HR) in a dose-dependent way, promoted H2O2 accumulation, and induced the expression of defense-relative genes in Nicotiana benthamiana. CONCLUSION The antiviral protein BLB8 from B. laterosporus strain B8 effectively inhibits TMV infection in inactivation, protective and curative effects through triggering plant immunity in tobacco. Therefore, the present study provides a new antiviral agent for prevention and control of viral disease.

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