Magnesium promotes tea plant growth via enhanced glutamine synthetase-mediated nitrogen assimilation

文献类型： 外文期刊

第一作者： Zhang, Qunfeng

作者： Zhang, Qunfeng;Shi, Yutao;Shi, Yuanzhi;Tang, Dandan;Ruan, Jianyun;Liu, Mei-Ya;Zhang, Qunfeng;Shi, Yuanzhi;Ruan, Jianyun;Liu, Mei-Ya;Shi, Yutao;Hu, Hao;Hu, Hao;Tang, Dandan;Fernie, Alisdair R.

作者机构：

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2023 年 192 卷 2 期

页码：

收录情况： SCI

摘要： Acidic tea (Camellia sinensis) plantation soil usually suffers from magnesium (Mg) deficiency, and as such, application of fertilizer containing Mg can substantially increase tea quality by enhancing the accumulation of nitrogen (N)-containing chemicals such as amino acids in young tea shoots. However, the molecular mechanisms underlying the promoting effects of Mg on N assimilation in tea plants remain unclear. Here, both hydroponic and field experiments were conducted to analyze N, Mg, metabolite contents, and gene expression patterns in tea plants. We found that N and amino acids accumulated in tea plant roots under Mg deficiency, while metabolism of N was enhanced by Mg supplementation, especially under a low N fertilizer regime. N-15 tracing experiments demonstrated that assimilation of N was induced in tea roots following Mg application. Furthermore, weighted gene correlation network analysis (WGCNA) analysis of RNA-seq data suggested that genes encoding glutamine synthetase isozymes (CsGSs), key enzymes regulating N assimilation, were markedly regulated by Mg treatment. Overexpression of CsGS1.1 in Arabidopsis (Arabidopsis thaliana) resulted in a more tolerant phenotype under Mg deficiency and increased N assimilation. These results validate our suggestion that Mg transcriptionally regulates CsGS1.1 during the enhanced assimilation of N in tea plant. Moreover, results of a field experiment demonstrated that high Mg and low N had positive effects on tea quality. This study deepens our understanding of the molecular mechanisms underlying the interactive effects of Mg and N in tea plants while also providing both genetic and agronomic tools for future improvement of tea production. Magnesium transcriptionally regulates the glutamine synthetase gene CsGS1.1 during the enhanced assimilation of nitrogen in tea plants.

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