Molecular characterization and function of hif1a and fih1 in response to acute thermal stress in American shad (Alosa sapidissima)

文献类型： 外文期刊

第一作者： Liang, Zhengyuan

作者： Liang, Zhengyuan;Hu, Songqin;Miao, Linghong;Zhu, Wenbin;Wang, Lanmei;Dong, Zaije;Liang, Zhengyuan;Dong, Yalun;Miao, Linghong;Zhu, Wenbin;Fu, Jianjun;Luo, Mingkun;Wang, Lanmei;Dong, Zaije;Dong, Yalun;Miao, Linghong;Zhu, Wenbin;Fu, Jianjun;Luo, Mingkun;Wang, Lanmei;Dong, Zaije;Feng, Bingbing

作者机构：

关键词： Alosa sapidissima; Hypoxia-inducible factor-1 alpha; Factor inhibiting hif1 alpha; Thermal stress; Housekeeping genes selection

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0920-1742

年卷期： 2024 年

页码：

收录情况： SCI

摘要： In order to evaluate the function of hypoxia-inducible factor-1 alpha (hif1 alpha) and factor inhibiting hif1 alpha (fih1) in response to thermal stress, we first conducted a functional analysis of A. sapidissima hif1 alpha and fih1, and determined hif1 alpha and fih1 expressions in different tissues in response to thermal stress based on identified housekeeping genes (HKGs). The results showed that hif1 alpha and fih1 were mainly located in the nucleus and cytoplasm. The full length cDNA sequence of hif1 alpha and fih1 was 4073 bp and 2759 bp, respectively. The cDNA sequence of hif1 alpha includes 15 exons encoding 750 amino acid residues, and the full length cDNA sequence of fih1 contains 9 exons encoding 354 amino acid residues. During the acute thermal stress transferring from 16 +/- 0.5 degrees C (control) to 20 +/- 0.5 degrees C, 25 +/- 0.5 degrees C, and 30 +/- 0.5 degrees C for 15 min, it was found that the expression trends of hif1 alpha and fih1 showed an inhibitory regulation in the heart, while they consistently expressed in brain, intestine, muscle, gill, kidney and liver. In conclusion, this is the first study to identify the tissue-specific HKGs in A. sapidissima and found that ef1 alpha and beta-actin are the most suitable HKGs. Hif1 alpha and Fih1 are mainly the nuclear and cytoplasmic proteins, respectively, having high levels in the heart and brain. Alosa sapidissima countered a temperature increase from 16 to 25 degree celsius by regulating the expressions of hif1 alpha and fih1, but their physiological regulatory functions were unable to cope with acute thermal stress when the temperature difference was 14 degree celsius (from 16 to 30 degree celsius).

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