Molecular Characterization of a Novel Cathepsin L in Macrobrachium nipponense and Its Function in Ovary Maturation

文献类型： 外文期刊

第一作者： Jiang, Sufei

作者： Jiang, Sufei;Xiong, Yiwei;Zhang, Wenyi;Gong, Yongsheng;Wu, Yan;Qiao, Hui;Fu, Hongtuo;Zhu, Junpeng;Cheng, Dan;Qiao, Hui;Fu, Hongtuo

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关键词： Macrobrachium nipponense; cathepsin L; ovary maturation; mRNA expression; RNA interference

期刊名称：FRONTIERS IN ENDOCRINOLOGY （ 影响因子：6.055； 五年影响因子：6.335 ）

ISSN： 1664-2392

年卷期： 2022 年 12 卷

页码：

收录情况： SCI

摘要： Cathepsin L genes, which belonged to cysteine proteases, were a series of multifunctional protease and played important roles in a lot of pathological and physiological processes. In this study, we analyzed the characteristics a cathepsin L (named Mn-CL2) in the female oriental river prawn, Macrobrachium nipponense which was involved in ovary maturation. The Mn-CL2 was1,582 bp in length, including a 978 bp open reading frame that encoded 326 amino acids. The Mn-CL2 was classified into the cathepsin L group by phylogenetic analysis. Real-time PCR (qPCR) analysis indicated that Mn-CL2 was highly expressed in the hepatopancreas and ovaries of female prawns. During the different ovarian stages, Mn-CL2 expression in the hepatopancreas and ovaries peaked before ovarian maturation. In situ hybridization studies revealed that Mn-CL2 was localized in the oocyte of the ovary. Injection of Mn-CL2 dsRNA significantly reduced the expression of vitellogenin. Changes in the gonad somatic index also confirmed the inhibitory effects of Mn-CL2 dsRNA on ovary maturation. These results suggest that Mn-CL2 has a key role in promoting ovary maturation.

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