Variation in cis-regulation of a NAC transcription factor contributes to tolerance in wheat

文献类型： 外文期刊

第一作者： Mao, Hude

作者： Mao, Hude;Li, Shumin;Chen, Bin;Mei, Fangming;Zhang, Yifang;Li, Fangfang;Chen, Nan;Du, Linying;Ding, Li;Wang, Zhongxue;Cheng, Xinxiu;Wang, Xiaojing;Kang, Zhensheng;Mao, Hude;Li, Shumin;Chen, Bin;Mei, Fangming;Zhang, Yifang;Li, Fangfang;Chen, Nan;Ding, Li;Wang, Zhongxue;Cheng, Xinxiu;Kang, Zhensheng;Mao, Hude;Kang, Zhensheng;Jian, Chao;Li, Tian;Du, Linying;Wang, Xiaojing;Kang, Zhensheng

作者机构：

关键词： wheat (Triticum aestivum); drought tolerance; GWAS; TaNAC071-A; cis-regulatory element; transcrip-tional regulation

期刊名称：MOLECULAR PLANT （ 影响因子：21.949； 五年影响因子：19.617 ）

ISSN： 1674-2052

年卷期： 2022 年 15 卷 2 期

页码：

收录情况： SCI

摘要： Drought is a major environmental factor limiting wheat production worldwide, and developing droughttolerant cultivars is a central challenge for wheat breeders globally. Therefore, it is important to identify genetic components determining drought tolerance in wheat. In this study, we identified a wheat NAC gene (TaNAC071-A) that is tightly associated with drought tolerance by a genome-wide association study. Knockdown of TaNAC071-A in wheat attenuated plant drought tolerance, whereas its overexpression significantly enhanced drought tolerance through improved water-use efficiency and increased expression of stress-responsive genes. This heightened water-saving mechanism mitigated the yield loss caused by water deficit. Further candidate gene association analysis showed that a 108-bp insertion in the promoter of TaNAC071-A alters its expression level and contributes to variation in drought tolerance among wheat accessions. This insertion contains two MYB cis-regulatory elements (CREs) that can be directly bound by the MYB transcription activator, TaMYBL1, thereby leading to increased TaNAC071-A expression and plant drought tolerance. Importantly, introgression of this 108-bp insertion allele, TaNAC071-AIn-693, into drought-sensitive cultivars could improve their drought tolerance, demonstrating that it is a valuable genetic resource for wheat breeding. Taken together, our findings highlight a major breakthrough in determining the genetic basis underlying phenotypic variation in wheat drought tolerance and showcase the potential of exploiting CRE-containing indels for improving important agronomical traits.

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