Proteomic Analysis of Differentially Expressed Plasma Exosome Proteins in Heat-Stressed Holstein cows

文献类型： 外文期刊

第一作者： Xia, Shuwen

作者： Xia, Shuwen;Jiang, Yingying;Li, Wenjie;An, Zhenjiang;Shen, Yangyang;Ding, Qiang;Chen, Kunlin;Xia, Shuwen;Shen, Yangyang;Ding, Qiang;Chen, Kunlin;Jiang, Yingying;Li, Wenjie;An, Zhenjiang

作者机构：

关键词： exosomes; heat stress; differentially expressed proteins; Holstein cows

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2025 年 15 卷 15 期

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收录情况： SCI

摘要： Heat stress in dairy cows, caused by high temperature and humidity during summer, has led to significant declines in milk production and severe economic losses for farms. Exosomes-extracellular vesicles carrying bioactive molecules-are critical for intercellular communication and immunity but remain understudied in heat-stressed Holstein cows. In this study, we extracted exosomes from three heat-stressed (HS) cows and three non-heat-stressed (Ctr) cows and employed proteomics to analyze plasma exosomes. We identified a total of 28 upregulated and 18 downregulated proteins in the HS group compared to the control group. Notably, we observed a significant upregulation of key protein groups, including cytoskeletal regulators, signaling mediators, and coagulation factors, alongside the downregulation of HP-25_1. These differentially expressed proteins demonstrate strong potential as heat stress biomarkers. GO and KEGG analyses linked the differentially expressed proteins to actin cytoskeleton regulation and endoplasmic reticulum pathways. Additionally, protein-protein interaction (PPI) analysis revealed the PI3K-Akt signaling pathway as a central node in the cellular response to heat stress. These findings establish plasma exosomes as valuable biospecimens, provide valuable insights into the molecular mechanisms of heat stress response, and may contribute to the development of precision breeding strategies for enhanced thermal resilience in dairy herds.

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