Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP

文献类型： 外文期刊

第一作者： Xiaoding Ma

作者： Xiaoding Ma;Chaozhu Xing;Liping Guo;Yangcang Gong;Hailin Wang;Yunlei Zhao;Jianyong Wu

作者机构：

关键词： cotton;cDNA-AFLP;gene expression;double-recessive GMS

期刊名称：Journal of Genetics and Genomics （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2007 年 34 卷 6 期

页码：

收录情况： CSCD

摘要： cDNA amplified fragment length polymorphism (cDNA-AFLP) analysis was used to investigate the differentially expressed genes between sterile and fertile plants of ms5ms6 double-recessive genic male sterility (GMS) two-type line cotton (Gossypium hirsutum L.) at different stages, i.e., sporogenous cell stage, pollen mother cell (PMC) stage, and pollen grain stage. Seventeen differentially expressed fragments were identified. Functional analysis indicated that their corresponding genes may participate in the processes of signal transduction, transcription, energy metabolism, and plant cell wall development. Northern blot demonstrated the credibility of the result of cDNA-AFLP. A sterility restorer factor-like gene, which only expressed in fertile anther and was notably homologous to T cytoplasm male sterility restorer factor 2 of maize (Zea mays L.), was identified in this research.

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