Homology-based characterization of the cis- regulatory elements modulate flavone induction of CYP321A1 in Helicoverpa armigera

文献类型： 外文期刊

第一作者： Deng, Zhongyuan

作者： Deng, Zhongyuan;Zhang, Yuting;Wang, Shan;Wang, Lixiang;Ding, Qian;Zhang, Yuting;Wang, Shan;Xie, Xingcheng;Xie, Xingcheng;Li, Xianchun;Xie, Xingcheng;Li, Xianchun;Ni, Xinzhi

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关键词： Helicoverpa; Flavone; Xenobiotic response element; Cytochrome P450; Mutation

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2024 年 204 卷

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收录情况： SCI

摘要： Xenobiotic response element (XRE) to flavone was the cis- regulatory elements that mediates the induction of the allelochemical-metabolizing CYP321A1 gene from Helicoverpa zea. However, it was unknown whether the XREFla element existed in other species. Recently we have identified and cloned the CYP321A1 gene with promoter region in a related species, Helicoverpa armigera. Sequence similarity of two orthologous CYP321A1 genes was 97.27%, but the promoter sequence similarity was only 56.32%. Sequence alignment showed the XRE-Fla like element owns three mutations in H. armigera compared with H. zea. Progressive 5 ' deletions and internal mutation indicated that H. armigera XRE-Fla was the essential element of CYP321A1 gene in response to flavone. XRE-Fla mutations and EMSA analysis confirmed that the H. armigera XRE-Fla element binding factor was stronger than H. zea. The findings indicate the XRE element mutations mainly contribute to the differences between the flavone-induced expressions of two CYP321A1 genes, which improve the flexibility and adaptability for allelochemical response of H. armigera.

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