Apoptosis, inflammatory and innate immune responses induced by infection with a novel goose astrovirus in goose embryonic kidney cells

文献类型： 外文期刊

第一作者： Hou, Zhanpeng

作者： Hou, Zhanpeng;Jin, Shaobing;Liang, Yu;Wang, Haiyue;Jiang, Danli;Cao, Nan;Tian, Yunbo;Liu, Wenjun;Xu, Danning;Fu, Xinliang;Hou, Zhanpeng;Jin, Shaobing;Liang, Yu;Wang, Haiyue;Jiang, Danli;Cao, Nan;Tian, Yunbo;Liu, Wenjun;Xu, Danning;Fu, Xinliang;Sun, Minhua

作者机构：

关键词： GoAstV; apoptosis; inflammatory response; innate immune; NLRP3

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：4.8； 五年影响因子：5.5 ）

ISSN： 2235-2988

年卷期： 2024 年 14 卷

页码：

收录情况： SCI

摘要： Introduction Since 2016, a highly lethal visceral gout induced by infection with the novel goose astrovirus (GoAstV) resulted in an ongoing outbreak in goslings in China, with a mortality rate ranging from 10% to 50%, and causing considerable economic losses in the goose industry. However, the pathogenesis of GoAstV and the molecular mechanism by which kidney lesions are induced by GoAstV infection are unclear.Methods In the present study, a GEK cell infection model for GoAstV was established, and the apoptosis, inflammatory and innate immune responses induced by GoAstV were investigated in GEK cells.Results The results shown that the expression of proapoptotic proteins, including Bax, caspase-3, caspase-9 and cytochrome c, increased in the infection group; however, the expression of the antiapoptotic protein Bcl-2 decreased, indicating that apoptosis was induced by GoAstV infection in GEK cells. Besides, the activation of the RIG-I/MDA5 pathway and the downstream upregulation of proinflammatory cytokines, including the adapter proteins MAVS, IRF7 and NF-kappa B and the proinflammatory cytokines IL-6, IL-8 and TNF-alpha, were detected in GEK cells infected with GoAstV. In addition, GoAstV infection induces the activation of the NLPR3 pathway and further stimulates the increased production of IL-1 beta. In summary, the present study revealed that GoAstV infection could induce apoptosis and the activation of the RIG-I/MDA5 and NLRP3 pathways in GEK cells, as well as the massive release of proinflammatory cytokines.Discussion These results are helpful for elucidating the molecular mechanism of pathological lesions in the kidney in gout goslings infected with GoAstV and the interaction between GoAstV and the innate immune system of the host.

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