Influence of CrylAb protein on growth and development of a predatory spider, Pardosa pseudoannulata, from protective perspectives

文献类型： 外文期刊

第一作者： He, Yuan

作者： He, Yuan;Chao, Yinying;Tang, Yun-e;Wang, Juan;Wang, Zhi;Peng, Yuan-de;Lv, Bo

作者机构：

关键词： CrylAb protein; Pardosa pseudocomulam; Enzyme activity; Tissue; Subcellular organelles; Transcriptome

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2024 年 269 卷

页码：

收录情况： SCI

摘要： The expression of Cry proteins in genetically modified rice varieties safeguards the crop from lepidopteran pests. These proteins have the potential to be transferred through the food chain to arthropods like planthoppers and predatory spiders, triggering defensive responses in these unintended organisms. Hence, we hypothesized that Cry protein might influence the growth and development of spiders by altering protective enzyme activities. The results showed that CrylAb protein could accumulate in tissues and subcellular organelles of Pardosa pseudoannulata from Nilaparvata lugens. CrylAb protein exposure prolonged the developmental duration in the 5th and 7th instar spiderlings but induced no alterations of other growth indicators, such as body length, median ocular area, and survival rate. In addition, CrylAb protein exerted no adverse impacts on several detoxifying enzymes (i.e., superoxide dismutase, catalase, glutathione peroxidase, and acetylcholine esterase) in muscle, midgut, ganglia, and hemolymph at subcellular components (i.e., microsome and cytoplasm). To further explore the effects of CrylAb protein on the spiderlings, we performed an integrated transcriptome analysis on spiderlings exposed to CrylAb protein. The results showed that CrylAb protein might prolong the development duration of P. pseudoannzdata via the altered cuticle metabolism (e.g., chitin metabolic process and structural constituent of cuticle). In addition, the gene expression profile associated with detoxifying enzymes and three stress-responsive pathways (JAK/STAT, JNK/SAPK, and Hippo pathways) also displayed no significant alterations under CrylAb exposure. Collectively, this integrated analysis generates multidimensional insights to assess the effects of CrylAb protein on non-target spiders and demonstrates that CrylAb protein exerts no toxicity in P. pseudoannulata.

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