Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers

文献类型： 外文期刊

第一作者： Zhang, Ruiying

作者： Zhang, Ruiying;Huang, Chenyang;Zheng, Suyue;Zhang, Jinxia;Ng, Tzi Bun;Jiang, Ruibo;Zuo, Xuemei;Wang, Hexiang

作者机构：

关键词： Lentinula edodes;ISSR;POLYMERASE CHAIN-REACTION;CULTIVATED STRAINS;AFLP;RAPD;GERMPLASM;PRIMERS;PCR;SSR;RFLP

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To validate strain typing by inter simple sequence repeat (ISSR) analysis in Lentinula edodes cultivars, 17 Chinese L. edodes strains including 15 cultivated strains cultivated on a large scale and two wild strains were analyzed with the ISSR technique. With the use of two ISSR primers, a total of 32 DNA products were detected, of which, 31 DNA products (96.9% of the detected products) were polymorphic between two or more strains. The profiles of those two primers could be employed to differentiate all of the tested strains. A cluster analysis based on ISSR data revealed that the 17 strains could be classified into two distinct groups. One group consisted of eight strains in which the cultivated strains were H (high-temperature)-type or B (broad-temperature)-type, and the other group comprised cultivated strains that were of the L (low-temperature)-type or M (medium-temperature)-type. In contrast to the two wild strains, the genetic diversity of 15 cultivated strains was very rich based on a similarity coefficient analysis.

分类号： Q93

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