Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes

文献类型： 外文期刊

第一作者： Qin, LH

作者： Qin, LH;Tan, Q;Chen, MJ;Pan, YJ

作者机构：

关键词： ISSR markers;SCAR markers;Lentinula edodes;edible mushroom;strain specificity;GENETIC DIVERSITY;POLYMORPHISMS;ISSR

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Although Lentinula edodes is the second most important cultivated mushroom worldwide, most industrially cultivated strains have been identified only through traditional phenotypic analysis. Here, we report for the first time the use of sequence characterized amplified region (SCAR) markers for strain differentiation. SCAR markers were created by first generating and sequencing single intersimple sequence repeats fragments, and then designing primers based on these sequences to amplify strain-specific fragments of a certain size. One SCAR primer pair, ISL450F/R-7 (amplifying a band of c. 450 bp), was designed to identify one strain of L. edodes (strain No. 7). The SCAR primer pair was then used to correctly amplify the single unique fragment from DNA samples taken from a total of 85 strains representing three separate species. Our data provide the foundation for a precise and rapid PCR-based strain-diagnostic system for L. edodes.

分类号： Q93

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