Cloning, Sequence and Expression Analysis of Gossypium barbadense L.pepc Gene

文献类型： 外文期刊

第一作者： Yu Jiwen

作者： Yu Jiwen;Yu Shuxun;Zhai Honghong;Fan Shuli;Song Meizhen;Zhang Jinfa

作者机构：

关键词： pepc;clone;differential expression;quantitative PCR;cotton

期刊名称：棉花学报

ISSN： 1002-7807

年卷期： 2011 年 23 卷 1 期

页码：

收录情况： CSCD

摘要： According to published EST sequences,an new pepc(Phosphoenolpyruvate carboxylase) gene was cloned from Gossypium barbadense L.cultivar 7124 by the utilization of the RACE and genome walking technology. The gene was named Gb.pepc3.It consists of 3259 bps, contains an open reading frame for 2910 bps, and encodes 969 amino acids with a deduced molecular weight of 110.7 kd and an isoelectric point of 6.08 I. Homology and phylogenetic trees analysis showed high similarity between Gb.pepc3 and pepcs in other reported plants, and all belong to C3 type pepc. The result of the fluorescence quantitative PCR displayed that Gb.pepc3 existed in all tissues of cotton. It expressed highly in the embryo but low in fiber. The expression quantity of Gb.pepc3 varied in different stages of the development of cotton. The expression of Gb.pepc3 reached the highest level at 15 days after blossoming for Gossypium barbadense L.while 20 days after blossoming for G.hirsutum, and there was significant difference in the expression quantities between two species

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