EXPRESSION OF THE BARLEY YELLOW DWARF VIRUS-GAV MOVEMENT PROTEIN AND ITS DETECTION IN THE INFECTED AND TRANSGENIC PLANTS

文献类型： 外文期刊

第一作者： Wang, X.

作者： Wang, X.;Liu, Y.;Peng, Y.;Zhou, G.

作者机构：

关键词： Barley yellow dwarf virus-GAV;movement protein;expression;immunization

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Movement proteins (MPs) that facilitate virus movement in the plants were identified in a number of plant viruses. In this study, full-length MP gene of the Chinese isolate Barley yellow dwarf virus-GAV (BYDV-GAV) was cloned and expressed in Escherichia coll. About 32% of the expressed MP was soluble providing the concentration of isopropyl-B-D-galactopyranoside (IPTG), time of the induction, temperature and shaking speed were optimized. The soluble MP was purified using nickel-affinity column. Immune serum prepared against purified MP was used for the detection of MP in the BYDV-GAV infected leaves of oat and in the leaves of transgenic wheat plants expressing the full-length and truncated MP gene.

分类号： R373

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