Transcriptome and Metabolome Analysis Reveals the Effect of Temperature on the Vegetative Mycelium of Morchella sextelata

文献类型： 外文期刊

第一作者： Hu, Xinyu

作者： Hu, Xinyu;Liu, Junhe;Wang, Zhen;Ma, Leran;Zhang, Tianyuan

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关键词： Morchella sextelata; transcriptome; metabolome; temperature; vegetative mycelium

期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）

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年卷期： 2024 年 10 卷 11 期

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收录情况： SCI

摘要： Morchella, a highly valued medicinal and edible mushrooms, is experiencing an increasing demand; however, its cultivation is significantly influenced by climatic conditions and soil characteristics. Consequently, elucidating the mechanisms underlying Morchella mycelium's response to temperature stress holds substantial importance for enhancing Morchella cultivation practices. In this study, we used Morchella sextelata as the research object and employed integrated transcriptomic and metabolomic analyses to evaluate the effects of cultivation temperatures set at 33 degrees C, 20 degrees C, and 4 degrees C on the vegetative mycelium of Morchella. Through these comprehensive analyses, we identified 2998 differentially expressed genes alongside 678 differentially accumulated metabolites. Utilizing Weighted Gene Co-expression Network Analysis (WGCNA), we constructed a co-expression network that revealed hub genes and metabolites within each module. Furthermore, through KEGG pathway analysis, we pinpointed significant metabolic pathways responsive to temperature stress-particularly those involved in purine metabolism, RNA degradation, two-component systems, ABC transporters, and pyruvate metabolism. Overall findings indicated that elevated temperatures exerted a more pronounced effect on M. sextelata mycelium compared to lower temperatures. These insights enhance our comprehension of the adaptive mechanisms of M. sextelata to thermal variations while providing valuable references for optimizing environmental temperature regulation in their cultivation as well as offering clues for selecting varieties capable of thriving under diverse thermal conditions.

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