Molecular characterization and functional analysis of a mollusk Rel homologous gene

文献类型： 外文期刊

第一作者： Chen, Jiwen

作者： Chen, Jiwen;Ma, Jilv;Cui, Jie;Dong, Juan;Qu, Yifan;Lu, Xiuqi;Wang, Anhao;Huang, Baoyu;Wang, Xiaotong;Zhang, Guoguang;Yu, Tao;Zheng, Yanxin;Cai, Shuai;Huang, Baoyu;Wang, Xiaotong

作者机构：

关键词： Scallop; Innate immunity; Rel; NF -KB; IKB

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 143 卷

页码：

收录情况： SCI

摘要： Members of the nuclear factor-kappa B (NF -KB) family are crucial regulators of physiological processes such as apoptosis, inflammation, and the immune response, acting as vital transcription factors to perform their function. In this study, we identified a NF -KB homologous gene (CfRel1) in Zhikong scallops. The 3006-bp-long open reading frame encodes 1001 amino acids. The N-terminus of the CfRel1 protein harbors a conserved Rel ho-mology domain (RHD) that contains a DNA-binding domain and a dimerization domain. According to the multiple sequence alignment results, both the DNA-binding and dimerization domains are highly conserved. Phylogenetic analysis indicated that CfRel1 is closely related to both the Dorsal protein of Pinctada fucata and the Rel2 protein of Crassostrea gigas. CfRel1 mRNA was expressed in all tissues tested in the quantitative reverse transcription PCR experiments, with hepatopancreatic tissue expressing the highest levels. Furthermore, after stimulation with lipopolysaccharide, peptidoglycan, or polyinosinic:polycytidylic acid, the mRNA expression level of CfRel1 was markedly increased. The co-immunoprecipitation test results showed that CfRel1 interacted with scallop IKB protein through its RHD DNA-binding domain, suggesting that IKB may regulate the activity of Rel1 by binding to this domain. Dual-luciferase reporter gene assays revealed that CfRel1 overexpression in HEK293T cells activated the activator protein 1 (AP-1), NF-KB, interferon (IFN)alpha, IFNfl, and IFN gamma reporter genes, indicating the diverse functions of the protein. In summary, CfRel1 is capable of responding to attacks from pathogen-associated molecular patterns, participating in immune signaling, and activating NF-KB and IFN re-porter genes. Our findings contribute to the advancement of invertebrate innate immunity theory, enrich the theory of comparative immunology, and serve as a reference for the future screening of disease-resistant strains in scallops.

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