The knockout of tobacco NtCBL10 inhibits leaf Cl- accumulation and leads to light-dependent necrosis & light-independent chlorosis under salt stress

文献类型： 外文期刊

第一作者： Mao, Jingjing

作者： Mao, Jingjing;Yuan, Guang;Liu, Haobao;Ning, Yang;Wang, Qian;Mao, Jingjing;Yuan, Guang;Mao, Jingjing;Visser, Richard G. F.;Bai, Yuling;van der Linden, C. Gerard;Mao, Jingjing;Mao, Jingjing;Xu, Gang;Xue, Lin;Mao, Dongping

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关键词： CBL10; Salt tolerance; Necrosis; Chlorosis; Light

期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 影响因子：4.7； 五年影响因子：5.4 ）

ISSN： 0098-8472

年卷期： 2025 年 229 卷

页码：

收录情况： SCI

摘要： CBL10 was shown to be a key gene for salt tolerance in Arabidopsis thaliana. In this study, we evaluated the role of CBL10 in the tobacco salt tolerance response by characterizing the gene editing-induced loss-of-function knockout mutants of the two NtCBL10 homeologous genes NtCBL10A and NtCBL10B. The importance of NtCBL10 for the response to salinity was evidenced by the salt supersensitivity of the Nt-cbl10a10b double mutants, with fast-developing chlorosis and severe necrotic lesions on leaves. Stomatal conductance and photochemical efficiency of photosystem 2 (PhiPS2) of the Nt-cbl10a10b double mutant were significantly inhibited already at a very early stage of the salt stress response. Leaf Na+ concentrations were not much affected in these plants, but the Cl- content of the Nt-cbl10a10b double mutants was significantly lower than that of wild-type plants, which is the first report of CBL10 in the regulation of Cl- homeostasis. Interestingly, the necrosis phenotype of Ntcbl10a10b double mutants was dependent on light, while the chlorosis phenotype of Nt-cbl10a10b double mutants was light-independent. Different from the previous studies that focus on the role of CBL10 in Na+ homeostasis regulation, this study indicates that NtCBL10 is a key component in regulating multiple aspects of ion homeostasis under salt stress.

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