eIF4A, a target of siRNA derived from rice stripe virus, negatively regulates antiviral autophagy by interacting with ATG5 in Nicotiana benthamiana
文献类型： 外文期刊
第一作者： Zhang, Xiangxiang
作者： Zhang, Xiangxiang;Yin, Yueyan;Su, Yunhe;Jia, Zhaoxing;Jiang, Liangliang;Lu, Yuwen;Zheng, Hongying;Peng, Jiejun;Rao, Shaofei;Wu, Guanwei;Chen, Jianping;Yan, Fei;Zhang, Xiangxiang;Yin, Yueyan;Chen, Jianping;Chen, Jianping
作者机构：
期刊名称：PLOS PATHOGENS （ 影响因子：6.823； 五年影响因子：7.455 ）
ISSN： 1553-7366
年卷期： 2021 年 17 卷 9 期
页码：
收录情况： SCI
摘要： Autophagy is induced by viral infection and has antiviral functions in plants, but the underlying mechanism is poorly understood. We previously identified a viral small interfering RNA (vsiRNA) derived from rice stripe virus (RSV) RNA4 that contributes to the leaf-twisting and stunting symptoms caused by this virus by targeting the host eukaryotic translation initiation factor 4A (eIF4A) mRNA for silencing. In addition, autophagy plays antiviral roles by degrading RSV p3 protein, a suppressor of RNA silencing. Here, we demonstrate that eIF4A acts as a negative regulator of autophagy in Nicotiana benthamiana. Silencing of NbeIF4A activated autophagy and inhibited RSV infection by facilitating autophagic degradation of p3. Further analysis showed that NbeIF4A interacts with NbATG5 and interferes with its interaction with ATG12. Overexpression of NbeIF4A suppressed NbATG5-activated autophagy. Moreover, expression of vsiRNA-4A, which targets NbeIF4A mRNA for cleavage, induced autophagy by silencing NbeIF4A. Finally, we demonstrate that eIF4A from rice, the natural host of RSV, also interacts with OsATG5 and suppresses OsATG5-activated autophagy, pointing to the conserved function of eIF4A as a negative regulator of antiviral autophagy. Taken together, these results reveal that eIF4A negatively regulates antiviral autophagy by interacting with ATG5 and that its mRNA is recognized by a virus-derived siRNA, resulting in its silencing, which induces autophagy against viral infection.
分类号：
- 相关文献
作者其他论文 更多>>
-
Promotion of symbiotic interaction between phagotrophic protists and beneficial bacteria, mediated via a balancing of soil nutrients, reduces the incidence of watermelon Fusarium wilt
作者：Tong, Yaoyao;Wang, Li;Wu, Chuanfa;Zhang, Haoqing;Chen, Jianping;Ge, Tida;Tong, Yaoyao;Wang, Li;Wu, Chuanfa;Zhang, Haoqing;Chen, Jianping;Ge, Tida;Tong, Yaoyao;Deng, Yangwu;Chen, Ming;Zheng, Xianqing;Lu, Weiguang
关键词：continuous cropping; co-occurrence network; Fusarium oxysporum; microbial community; nitrogen limitation; soil-borne pathogen
-
Bio-organic fertilizer enhances soil mineral solubilization, microbial community stability, and fruit quality in an 8-year watermelon continuous cropping system
作者：Tong, Yaoyao;Deng, Yangwu;Chen, Ming;Tong, Yaoyao;Zhang, Haoqing;Chen, Jianping;Ge, Tida;Yuan, Zhaofeng;Zheng, Xianqing;Lv, Weiguang;Liu, Hongwei;Liu, Hongwei;Chen, Jianping;Ge, Tida;Yuan, Zhaofeng
关键词：Successive cropping obstacle; Soil available iron; Microbial stability; Potential mineral solubilizing bacteria
-
Genome-Wide Identification and Comprehensive Characterization of Luffa Sucrose Phosphate Synthase Gene Family and Revealing LaSPS3/4's Role in Drought Resistance
作者：Tian, Xiaocheng;He, Guoliang;Wang, Hanyi;Huang, Liujing;Yin, Yeqiu;Wang, Guoping;Zhao, Hongbo;Tian, Xiaocheng;Ma, Chongjian;Tian, Xiaocheng;Sun, Shaolong;Liu, Jianting;Zhu, Haisheng;Yan, Fei
关键词：
Luffa acutangula ;SPS ; bioinformatics analysis -
An orphan viral genome with unclear evolutionary status sheds light on a distinct lineage of flavi-like viruses infecting plants
作者：Xu, Zhongtian;Li, Yiyuan;Sun, Zongtao;Chen, Jianping;Zhang, Chuanxi;Li, Junmin;Wang, Xifeng;Zheng, Luping;Gao, Fangluan
关键词：Snake River Alfalfa virus; flaviviridae; endornaviridae; flavi-like virus
-
Genome-wide analysis of CHYR gene family and BnA03.CHYR.1 functional verification under salt stress in Brassica napus L.
作者：Guo, Yanli;Liu, Fei;Wan, Heping;Ren, Qingxiao;Song, Manman;Zhang, Xiangxiang
关键词：
Brassica napus ; CHYR; Abiotic stress; Ubiquitin E3 ligase;BnA03.CHYR.1 -
The phytohormone jasmonic acid is involved in rice resistance to Southern rice black-streaked dwarf virus
作者：Huang, Chaorui;Qi, Qingling;Gao, Chenfei;Li, Lulu;Li, Yanjun;Chen, Jianping;Sun, Zongtao;Zhang, Hehong;Liu, Qing;Xu, Jianlong
关键词：Oryza sativa (rice); Jasmonic acid; Southern rice black-streaked dwarf virus; (SRBSDV); Variety resistance; Defense response
-
Identification and comparative transcriptome analyses between resistant and susceptible soybean varieties in response to soybean mosaic virus and cowpea mild mottle virus infection
作者：Li, Huajuan;Xu, Manru;Shang, Jiahui;Feng, Xiuli;Chen, Jianping;Sun, Zongtao;Wei, Zhongyan;Dong, Zhimin
关键词：Soybean mosaic virus (SMV); Cowpea mild mottle virus (CPMMV); Soybean; Variety resistance; RNA sequencing (RNA-seq)
