Fine mapping of the fiber length-related qFL-A12-2 QTL through the use of chromosome segment substitution lines and candidate gene validation

文献类型： 外文期刊

第一作者： Xiao, Xianghui

作者： Xiao, Xianghui;Liu, Ruixian;Gong, Juwu;Li, Pengtao;Zhang, Haibo;Chen, Yu;Sun, Zhihao;Peng, Renhai;Lu, Quanwei;Gong, Juwu;Gong, Wankui;Liu, Aiying;Ge, Qun;Liu, Yangming;Shang, Haihong;Li, Junwen;Yan, Haoliang;Pan, Jingtao;Shi, Yuzhen;Yuan, Youlu;Gong, Juwu;Yang, Rui;Wang, Yongbo;Gong, Juwu

作者机构： Anyang Inst Technol, Sch Biotechnol & Food Engn, Anyang 455000, Peoples R China;Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang 455000, Peoples R China;Xinjiang Prod & Construct Corps Seventh Div Agr Re, Kuitun, Peoples R China;Cotton Sci Res Inst Hunan, Changde 415101, Peoples R China

关键词： Single segment introgression line; Fiber length; BSA; Fine mapping; GhALMT12_A12

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 2023影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 220 卷

页码：

收录情况： SCI

摘要： Fiber length (FL) is one of the primary factors used to determine the quality of cotton fibers, serving as a primary target for the domestication and breeding of cotton plants through artificial selection. While many studies have identified quantitative trait loci (QTLs) associated with fiber length, few efforts have explored the mechanisms underlying the development of cotton fibers through fine mapping or the validation of related candidate genes. In a previous study, qFL-A12 - 2 was identified as a QTL on chromosome A12 that was associated with higher levels of fiber quality in the MBI7747 (BC 4 F 3:5 ) chromosome segment substitution line (CSSL). For fine mapping the QTL, a single-segment substitution line (CSSL-023) screened from BC 5 F 2 was backcrossed with the recurrent parental CCRI45 line to establish a large segregation population. Subsequently, 2092 individual BC 6 F 2 specimens were utilized in a fine-mapping effort employing highly dense simple sequence markers, which narrowed qFLA12 - 2 to a 0.65 Mb genomic region in Gossypium hirsutum containing 12 annotated genes. The most promising candidate gene within this interval was identified through qRT-PCR and complete coding sequence comparative analyses as GhALMT12_A12 , which encodes an aluminum-activated malate transporter. Two non-synonymous mutations were identified when the protein-coding portions of GhALMT12_A12 were compared among the Hai1, MBI7747, and CCRI45 varieties. Verification of GhALMT12_A12 silencing by VIGS in cotton revealed that the FL of silenced plants was significantly shorter than that of the control plants. When overexpressed, GhALMT12_A12 significantly enhanced Arabidopsis resistance to salt stress and drought conditions through altering ion transport. The outcomes underscore the notable function of GhALMT12_A12 in developing cotton fibers, providing a fundamental basis for scholars aiming at augmenting the length of cotton fibers.

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