Identification, characteristics and whole genome analysis of a pathogenic Aeromonas hydrophila ST2616 from diseased Asian swamp eel (Monopterus albus) with hemorrhagic sepsis

文献类型： 外文期刊

第一作者： Wang, Yujie

作者： Wang, Yujie;Qiu, Junqiang;Wang, Yunyan;Zhou, Zebin;Zhang, Yunru;Li, Mingyou;Zhang, Xiaolong;Chen, Xi;Ayana, Gelana Urgesa;Zhang, Qinghua;Wang, Yujie;Qiu, Junqiang;Wang, Yunyan;Zhou, Zebin;Zhang, Yunru;Li, Mingyou;Zhang, Xiaolong;Chen, Xi;Ayana, Gelana Urgesa;Zhang, Qinghua;Wang, Yujie;Qiu, Junqiang;Wang, Yunyan;Zhou, Zebin;Zhang, Yunru;Li, Mingyou;Zhang, Xiaolong;Chen, Xi;Ayana, Gelana Urgesa;Zhang, Qinghua;Yuan, Quan;Zhou, Wenzong

作者机构：

关键词： Asian swamp eel ( Monopterus albus ); Aeromonas hydrophila; Whole genome sequencing; Virulence gene; Antibiotics resistance gene

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2352-5134

年卷期： 2025 年 42 卷

页码：

收录情况： SCI

摘要： This study reports the cause of mass mortality in diseased Asian swamp eel (Monopterus albus) with hemorrhagic sepsis in Shanghai. A pathogenic strain of Aeromonas hydrophila MaAh001 was isolated from the blood of diseased eels. Experimental infection confirmed that MaAh001 is the pathogen responsible for mass mortality event. Histopathological examinations revealed this strain caused different tissue damage. The median lethal dose (LD50) of MaAh001 was determined as 3.16 x 107 CFU/mL, which is converted to an LD50 per unit weight of 1.58 x 106 CFU/g. Virulence gene test and antibiotic resistance detection indicated that MaAh001 carried 14 virulence factor and four antibiotics resistance genes. Drug sensitivity test showed that MaAh001 was highly sensitive to 16 antibiotics. Whole genome sequencing indicated that the strain has a total length of 4, 893 Mb, the G+C content of 61.2 %, and is registered in the GenBank under the entry number CP141267.1. According to the multilocus sequence typing (MLST) analysis, MaAh001 belonged to A. hydrophila ST2616 (PubMLST database id: 3152). Comparison with the pathogen virulence factor database (VFDB) indicated that this strain carried a variety of virulence genes related to adhesion, invasion and secretion systems. Analysis results of the comprehensive antibiotic resistance database (CARD) showed that MaAh001 carried the most common five beta-lactam resistance genes, including cphA2, OXA-726, CepS, P26918 and YP_857635. In conclusion, this study reported a typical case of A. hydrophila MaAh001 strain infection in eels, which laid a theoretical foundation for the prevention and treatment of eel sepsis in aquaculture and the development of subsequent vaccines.

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