Protective Effect of l-Theanine on Cyclophosphamide-Induced Testicular Toxicity in Mice

文献类型： 外文期刊

第一作者： Ye, Yulong

作者： Ye, Yulong;Fang, Chunyan;Li, Lanying;Liu, Dongna;Wang, Yingchun;Huang, Fan;Gong, Xuejiao;Xu, Yaqiong;Yao, Yu;Luo, Fan;Fang, Chunyan;Ye, Shanrong;Feng, Dejian

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关键词： l-theanine; cyclophosmamide; proteomics; metabolomics; testis toxicity

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 21 期

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收录情况： SCI

摘要： l-Theanine is the most abundantfree amino acid presentin tea. Several tea components have been studied for their impacton male fertility, but little is known about the effects of l-theanine. Cyclophosphamide (CP) is an antineoplastic and immunosuppressiveagent that reduces fertility in males. In the present study, we evaluatedthe effect of l-theanine on CP-induced testicular toxicityin male mice. A single dosage of 50 mg/kg saline or CP was administeredintraperitoneally over the course of 5 days. Mice were administered l-theanine (80 mg/kg) or saline by gavage for 30 days. Animalswere euthanized 24 h after the last l-theanine administration,and the testes were removed for histopathological and transmissionelectron microscopy analysis. Histological evaluation and transmissionelectron microscopy showed that administration of l-theaninealleviated CP-induced damage to the testicles, including spermatogonialcells, epithelial cells, seminiferous tubules, and basement membrane.An integrated proteomics and metabolomics investigation of testesrevealed that l-theanine therapy substantially affected thequantity of 719 proteins (395 upregulated and 324 downregulated) and196 metabolites (75 upregulated and 111 downregulated). The top threeenriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways forthese proteins and metabolites were purine metabolism, choline metabolismin cancer, and arachidonic acid metabolism. This is the first studyto reveal the protective effect of l-theanine on CP-inducedtesticular toxicity. l-Theanine could be a potential naturalactive substance for resistance to the testis toxicity induced byCP.

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