Deleting the C84L Gene from the Virulent African Swine Fever Virus SY18 Does Not Affect Its Replication in Porcine Primary Macrophages but Reduces Its Virulence in Swine

文献类型： 外文期刊

第一作者： Yang, Jinjin

作者： Yang, Jinjin;Ke, Junnan;Wang, Yu;Qian, Aidong;Yang, Jinjin;Zhu, Rongnian;Zhang, Yanyan;Zhou, Xintao;Yue, Huixian;Li, Qixuan;Miao, Faming;Chen, Teng;Zhang, Fei;Zhang, Shoufeng;Hu, Rongliang;Yang, Jinjin;Zhu, Rongnian;Zhang, Yanyan;Zhou, Xintao;Yue, Huixian;Li, Qixuan;Miao, Faming;Chen, Teng;Zhang, Fei;Zhang, Shoufeng;Hu, Rongliang

作者机构：

关键词： African swine fever virus (ASFV); C84L; deletion; virulence; replication

期刊名称：PATHOGENS （ 影响因子：3.7； 五年影响因子：3.7 ）

ISSN：

年卷期： 2024 年 13 卷 2 期

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收录情况： SCI

摘要： (1) Background: African swine fever (ASF) is a highly contagious disease that causes high pig mortality. Due to the absence of vaccines, prevention and control are relatively challenging. The pathogenic African swine fever virus (ASFV) has a complex structure and encodes over 160 proteins, many of which still need to be studied and verified for their functions. In this study, we identified one of the unknown functional genes, C84L. (2) Methods: A gene deficient strain was obtained through homologous recombination and several rounds of purification, and its replication characteristics and virulence were studied through in vitro and in vivo experiments, respectively. (3) Results: Deleting this gene from the wild-type virulent strain SY18 did not affect its replication in porcine primary macrophages but reduced its virulence in pigs. In animal experiments, we injected pigs with a 102 TCID50, 105 TCID50 deletion virus, and a 102 TCID50 wild-type strain SY18 intramuscularly. The control group pigs reached the humane endpoint on the ninth day (0/5) and were euthanized. Two pigs in the 102 TCID50(2/5) deletion virus group survived on the twenty-first day, and one in the 105 TCID50(1/5) deletion virus group survived. On the twenty-first day, the surviving pigs were euthanized, which was the end of the experiment. The necropsies of the survival group and control groups' necropsies showed that the surviving pigs' liver, spleen, lungs, kidneys, and submaxillary lymph nodes did not show significant lesions associated with the ASFV. ASFV-specific antibodies were first detected on the seventh day after immunization; (4) Conclusions: This is the first study to complete the replication and virulence functional exploration of the C84L gene of SY18. In this study, C84L gene was preliminarily found not a necessary gene for replication, gene deletion strain SY18 Delta C84L has similar growth characteristics to SY18 in porcine primary alveolar macrophages. The C84L gene affects the virulence of the SY18 strain.

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