Identification and Fine-Mapping of Clubroot (Plasmodiophora brassicae) Resistant QTL in Brassica rapa

文献类型： 外文期刊

第一作者： Zhang, Hui

作者： Zhang, Hui;Ma, Xiaochao;Liu, Xitong;Zhang, Shifan;Li, Fei;Li, Guoliang;Sun, Rifei;Zhang, Shujiang

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关键词： Plasmodiophora brassicae; Brassica rapa; QTL; resistant genes; BSA-Seq; fine-mapping

期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）

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年卷期： 2022 年 8 卷 1 期

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收录情况： SCI

摘要： European fodder turnips (Brassica rapa ssp. rapifera) were identified as sources of clubroot resistance (CR) and have been widely used in Brassica resistance breeding. An F-2 population derived from a cross between a resistant turnip and a susceptible Chinese cabbage was used to determine the inheritance and locating the resistance Quantitative Trait Loci (QTLs). The parents showed to be very resistant/susceptible to the field isolates (pathotype 4) of clubroot from Henan in China. After inoculation, 27 very resistant or susceptible individuals were selected to construct bulks, respectively. Next-generation-sequencing-based Bulk Segregant Analysis Sequencing (BSA-Seq) was used and located resistance QTL on chromosome A03 (3.3-7.5 Mb) and A08 (0.01-6.5 Mb), named Bcr1 and Bcr2, respectively. Furthermore, an F-3 population including 180 families derived from F-2 individuals was phenotyped and used to verify and narrow candidate regions. Ten and seven Kompetitive Allele-Specific PCR (KASP) markers narrowed the target regions to 4.3-4.78 Mb (A03) and 0.02-0.79 Mb (A08), respectively. The phenotypic variation explained (PVE) of the two QTLs were 33.3% and 13.3% respectively. The two candidate regions contained 99 and 109 genes. In the A03 candidate region, there were three candidate R genes, namely Bra006630, Bra006631 and Bra006632. In the A08 candidate region, there were two candidate R genes, namely Bra030815 and Bra030846.

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