miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ)

文献类型： 外文期刊

第一作者： Zhang, Yong-Hong

作者： Zhang, Yong-Hong;Xie, Heng-Li;Yang, Yu-Wei;Zhang, Jia-Bao;Wen, Jie;Liu, Ran-Ran;Zhao, Gui-Ping;Tan, Xiao-Dong;Liu, Zhen;Zheng, Yi

作者机构：

关键词： chicken; fatty liver; miR-375; RBPJ; lipid metabolism

期刊名称：POULTRY SCIENCE （ 影响因子：4.014； 五年影响因子：4.192 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 1 期

页码：

收录情况： SCI

摘要： Poultry is susceptible to fatty liver which lead to decrease egg production and increase mortality. But the potential molecular mechanisms remain largely unclear. In the current study, in combination with tran-scriptome sequencing and miRNA sequencing data anal-ysis from F1 generation of the normal liver and fatty liver tissues, the differentially expressed miR-375 and its target gene RBPJ were screened and verified. The expression levels of miR-375 and RBPJ gene in the liver between control and fatty liver groups of F0-F3 genera-tion for Jingxing-Huang (JXH) chicken are different significantly (P < 0.05 or P < 0.01). And downregu-lated RBPJ expression can promote TG content and lipid droplets in primary hepatocytes cultured in vitro (P < 0.01). Cell proliferation-related genes, including PMP22, IGF-1, IGF-2, and IGFBP-5, increased or decreased significantly after overexpression or knock-down RBPJ (P < 0.05 or P < 0.01), respectively. This study uniquely revealed that miR-375 induced lipid synthesis and inhibited cell proliferation may partly due to regulation of RBPJ expression, thereby involv-ing in fatty liver formation and inheritance in chicken. The results could be useful in identifying candidate genes and revealing the pathogenesis of fatty liver that may be used for disease-resistance selective breeding in chicken.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序