文献类型： 外文期刊
第一作者： You, Xiaoxiao
作者： You, Xiaoxiao;Fang, Qinyuan;Zhang, Teng;Zhou, Yang;Wang, Zheng;Sun, Tianhao;Zhou, Huanmin;Dai, Yanfeng;Liu, Yongbin;Chen, Chunhai;Yang, Xukui;Jian, Jianbo;Cao, Junwei;Wang, Shenyuan;Zhou, Huanmin;Fu, Shaoyin;He, Xiaolong;He, Jiangfeng;Wang, Biao;Wang, Liwei;Te, Rigele;Liu, Yongbin
作者机构：
期刊名称：SCIENTIFIC DATA （ 影响因子：5.8； 五年影响因子：8.9 ）
ISSN：
年卷期： 2024 年 11 卷 1 期
页码：
收录情况： SCI
摘要： Advancements in sequencing have enabled the assembly of numerous sheep genomes, significantly advancing our understanding of the link between genetic variation and phenotypic traits. However, the genome of East Friesian sheep (Ostfriesisches Milchschaf), a key high-yield milk breed, remains to be fully assembled. Here, we constructed a near-complete and gap-free East Friesian genome assembly using PacBio HiFi, ultra-long ONT and Hi-C sequencing. The resulting genome assembly spans approximately 2.96 Gb, with a contig N50 length of 104.1 Mb and only 164 unplaced sequences. Remarkably, our assembly has captured 41 telomeres and 24 centromeres. The assembled sequence is of high quality on completeness (BUSCO score: 97.1%) and correctness (QV: 69.1). In addition, a total of 24,580 protein-coding genes were predicted, of which 97.2% (23,891) carried at least one conserved functional domain. Collectively, this assembly provides not only a near T2T gap-free genome, but also provides a valuable genetic resource for comparative genome studies of sheep and will serve as an important tool for the sheep research community.
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