Purification-induced damage to calicivirus particles at near-atomic resolution

文献类型： 外文期刊

第一作者： Qu, Zehui

作者： Qu, Zehui;Meng, Kaiwen;Zhang, Yueping;Meng, Geng;Qu, Zehui;Kang, Hongtao;Qu, Liandong;Cui, Chenxi;Zhang, Xinzheng;Cui, Chenxi;Zhang, Xinzheng

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关键词： Feline calicivirus; virus flexibility evaluation; cryo-EM

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：5.141； 五年影响因子：4.169 ）

ISSN： 0022-1317

年卷期： 2022 年 103 卷 5 期

页码：

收录情况： SCI

摘要： The purification of virus particles is an essential process for the manufacture of vaccines. However, the application of different purification processes may affect the quality of the virus particles, such as structural integrity and homogeneity, which may further influence the infectivity and immunogenicity of the purified virus. In this study, we took Feline calicivirus (FCV). a common natural pathogen in cats belonging to Caliciviridae, as a research model. By using cryo-electron microscopy (cryo-EM), we incorporated the 3D classification process as a virus flexibility evaluation system. Cryo-EM images of virus particles resulting from different purification processes were compared at near-atomic resolution. The results indicated that molecular sieving purification will impact the stability of P-domains through increasing flexibility as determined by the evaluation system. which can be extended to assess the purification effect on the entire particle. This evaluation process can be further applied to all non-enveloped viruses.

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