P-solubilising bacteria and P-cycling genes drive soil P transformation during green manure decomposition

文献类型： 外文期刊

第一作者： Khan, Adnan Anwar

作者： Khan, Adnan Anwar;Chen, Yupei;Asghar, Rana Muhammad Ammar;Shah, Tahir;Zhang, Dabin;Gao, Yajun;Azeem, Imran;Cao, Weidong;Zhang, Dabin;Gao, Yajun

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关键词： Cover crop; P fractions; P-solubilising bacteria; P -cycling genes; Enzyme activity

期刊名称：AGRICULTURE ECOSYSTEMS & ENVIRONMENT （ 影响因子：6.4； 五年影响因子：6.8 ）

ISSN： 0167-8809

年卷期： 2025 年 394 卷

页码：

收录情况： SCI

摘要： Soil phosphorus (P) fixation and limited availability result in substantial challenges in agroecosystems. The use of green manure (GM) may enhance P availability; however, the temporal evolution of soil P fractions, P-solubilising bacteria (PSB) and P-cycling genes (PCGs) during GM decomposition remains unclear. Herein, we performed a 360-day field experiment to investigate the biomass decomposition rate and P release from the shoots and roots of rapeseed (RS), black bean (BB) and their mixture (Mix). Hedley's fractionation method, 16S rRNA gene amplicon sequencing and functional prediction using PICRUSt2 were used to evaluate soil P fractions, the relative abundance of PSB and the relative abundance of PCGs, respectively, on days 5, 120 and 360 after GM incorporation. The shoots and roots decomposition and P release rates were higher in RS than in BB and Mix (p < 0.05). After 120 days, the total labile P content in soil incorporated with RS shoots and roots remarkably increased by 2.9- and 3.5-fold, respectively (p < 0.05). The higher relative abundance of PSB, such as Xanthomonas, Pseudomonas, Haliangium, Bradyrhizobium, Pirellula and Sphingomonas, likely enhanced labile P content in soil. Similarly, the relative abundance of genes related to P-transportation (phnD and PhnE), P regulation (phoB, phoR and phoU), organic P (P-o) mineralisation (phnA, phnP, phoA, phoD and uqpQ) and inorganic P (P-i) solubilisation (gcd, ppa and ppx) were higher on day 120. Partial least squares path modelling (PLS-PM) showed that PSB and PCGs positively influenced soil enzyme activities on day 120, thereby enhancing the biochemical processes and promoting P transformation into labile forms. Redundancy analysis (RDA) showed that the increase in labile P content was mainly attributed to a decrease in non-labile P (concentrated HCl-P-o) content, as evidenced by their negative correlation. These findings suggest that GM crops, especially RS, can enhance the abundance of beneficial PSB and PCGs, driving the conversion of P-o to available forms. Therefore, GM is a promising sustainable strategy for improving soil P availability in agroecosystems.

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