Transcriptome of Kurthia gibsonii TYL-A1 Revealed the Biotransformation Mechanism of Tylosin

文献类型： 外文期刊

第一作者： Wang, Ye

作者： Wang, Ye;Zhao, Cuizhu;Zhao, Boyu;Duan, Xinran;Hao, Peng;Yang, Lianyu;Gao, Yunhang;Liang, Xiaojun

作者机构：

关键词： tylosin; antioxidant reaction; degradation; transcriptome sequencing

期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN：

年卷期： 2024 年 12 卷 12 期

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收录情况： SCI

摘要： Tylosin (TYL) pollution has aroused widespread concern, and its existence poses a serious threat to the environment and human health. Microbial degradation of antibiotics is considered to be an effective strategy to reduce the environmental impact of antibiotics, but its degradation mechanism is still unclear. In this study, transcriptome analysis was combined to explore the response mechanism of K. gibsonii strain TYL-A1 under TYL stress. The results showed that the strain showed a significant antioxidant response under TYL stress to cope with TYL-induced cell damage. TYL also increased the level of intracellular reactive oxygen species (ROS), damaged the integrity of the cell membrane, and inhibited the growth of strain TYL-A1. Transcriptome sequencing showed that under TYL exposure conditions, 1650 DEGs in strain TYL-A1 showed expression changes, of which 806 genes were significantly up-regulated and 844 genes were significantly down-regulated. Differentially expressed DEGs were significantly enriched in pathways related to metabolism, biosynthesis, and stress response, and tricarboxylic acid cycle, oxidative phosphorylation, and carbon metabolism genes were significantly up-regulated. In conclusion, this study provides novel insights regarding the degradation of TYL by K. gibsonii TYL-A1.

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