Genome-wide association of single nucleotide polymorphism loci and candidate genes for frogeye leaf spot (Cercospora sojina) resistance in soybean

文献类型： 外文期刊

第一作者： Gu, Xin

作者： Gu, Xin;Zhu, Zhiguo;Gu, Xin;Yang, Xiaohe;Yao, Liangliang;Gao, Xuedong;Zhang, Maoming;Liu, Wei;Qiu, Lei;Zhao, Haihong;Wang, Qingsheng;Li, Zengjie;Li, Zhimin;Meng, Qingying;Ding, Junjie;Gu, Xin;Yang, Xiaohe;Yao, Liangliang;Gao, Xuedong;Zhang, Maoming;Liu, Wei;Qiu, Lei;Zhao, Haihong;Wang, Qingsheng;Li, Zengjie;Li, Zhimin;Meng, Qingying;Ding, Junjie;Huang, Shanshan;Ma, Yansong;Wang, Chao;Hu, Xiping;Yang, Shuai

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关键词： Soybean; Cercospora sojina; Genome-wide association study; Resistant haplotype; Frogeye leaf spot resistant genes

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.26； 五年影响因子：5.761 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Frogeye leaf spot (FLS) is a destructive fungal disease that affects soybean production. The most economical and effective strategy to control FLS is the use of resistant cultivars. However, the use of a limited number of resistant loci in FLS management will be countered by the emergence of new high-virulence Cercospora sojina races. Therefore, we identified quantitative trait loci (QTL) that control resistance to FLS and identified novel resistant genes using a genome-wide association study (GWAS) on 234 Chinese soybean cultivars. Results A total of 30,890 single nucleotide polymorphism (SNP) markers were used to estimate linkage disequilibrium (LD) and population structure. The GWAS results showed four loci (p < 0.0001) distributed over chromosomes (Chr.) 5 and 20, that are significantly associated with FLS resistance. No previous studies have reported resistance loci in these regions. Subsequently, 45 genes in the two resistance-related haplotype blocks were annotated. Among them, Glyma20g31630 encoding pyruvate dehydrogenase (PDH), Glyma05g28980, which encodes mitogen-activated protein kinase 7 (MPK7), and Glyma20g31510, Glyma20g31520 encoding calcium-dependent protein kinase 4 (CDPK4) in the haplotype blocks deserves special attention. Conclusions This study showed that GWAS can be employed as an effective strategy for identifying disease resistance traits in soybean and narrowing SNPs and candidate genes. The prediction of candidate genes in the haplotype blocks identified by disease resistance loci can provide a useful reference to study systemic disease resistance.

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