qTaHa-5DL: another major QTL regulating wheat grain hardness

文献类型： 外文期刊

第一作者： Fan, Xiangyun

作者： Fan, Xiangyun;Jiang, Peng;He, Yi;Zhang, Xu;Yu, Guihong;Zhang, Peng;Fan, Xiangyun;Jiang, Peng;He, Yi;Zhang, Peng;Zhou, Meixue

作者机构：

关键词： Common wheat; Grain hardness; GWAS; QTL; KASP marker

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Grain hardness has important effects on grain quality and the end-use of wheat. In this study, a collection of 103 common wheat germplasms and a DH population of 194 lines were used to identify new quantitative trait loci (QTL) for grain hardness. Two stable genetic loci on chromosome 5D were detected under different locations and years with one of them being the Ha locus on 5DS where the major gene Puroindolines for wheat grain hardness is located. Another locus of qTaHa-5DL also showed a significant impact on grain hardness index (HI) with HI increasing from similar to 20 to similar to 45 and hardness type changing from soft to mixed when Puroindolines are wild type. A kompetitive allele-specific PCR (KASP) marker for qTaHa-5DL was developed and the effectiveness of the QTL was confirmed in 184 breeding lines with the marker K-Ha5DL dividing wheat into two distinct categories. This new QTL can be effectively used to select soft or medium hard wheat.

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